Lipid biosynthesis in microalgae can be stimulated by cultivation in low nitrogen medium. MACC-401 was isolated from the soil surface in Tres Marias (MG-Brazil). The strain shows the morphological characteristics of the Scenedesmaceae green algae. The daily biomass and lipid production of MACC-401 is remarkable, 0.36 g L⁻¹ and 110 mg L⁻¹, respectively. Exploration of the genetic background of this promising strain not only allows the utilization of this species for industrial-scale lipid production, but also provides genetic targets to select lipid-producing strains from microalgae collections. We conducted physiological experiments by cultivating MACC-401 in complete and N-limited media and performed genome sequencing as well as transcriptome analysis. The estimated nuclear genome size of MACC-401 is 99.503 Mbp and the chloroplast genome is 0.15 Mbp. The phylogenetic analysis confirmed that the MACC-401 belongs to the Scenedesmaceae family, and represents a genetically distinct accession in this family. A basic comparative transcriptome analysis resulted in the identification of N-starvation responsive genes, which could serve as markers to monitor the onset of lipid accumulation in algal cultures.

通过在低氮培养基中培养可以刺激微藻中的脂质生物合成。从Tres Marias（MG-Brazil）的土壤表面分离出MACC-401。该菌株显示了场面藻科绿藻的形态特征。MACC-401的每日生物量和脂质产量显着，分别为0.36 g L ^-1和110 mg L ^-1， 分别。对这种有前途的菌株的遗传背景的探索不仅允许将该物种用于工业规模的脂质生产，而且还提供了遗传靶标以从微藻集合中选择产生脂质的菌株。我们通过在完全和N受限培养基中培养MACC-401进行了生理学实验，并进行了基因组测序和转录组分析。MACC-401的估计核基因组大小为99.503 Mbp，叶绿体基因组为0.15 Mbp。系统发育分析证实，MACC-401属于风叶科，并且是该家族中遗传上不同的种。基本的比较转录组分析结果鉴定出了N饥饿反应基因，