This study investigates the cellulases-catalyzed hydrolysis of newspaper waste and paper slurries as carbon source in combination with digestate from anaerobic digestion as nitrogen source for the heterotrophic cultivation of Chlorella sorokiniana. The enzymatic hydrolysis of paper slurries resulted in a hydrolysate containing 27.2 g l⁻¹ glucose reflecting a 78% cellulose conversion. A maximum Chlorella sorokiniana biomass yield of 3.64 g l⁻¹ was achieved within 91 h of cultivation under consumption of 9.7 g l⁻¹ glucose. The most efficient cultivation converted 1 g l⁻¹ glucose into 0.42 g l⁻¹ biomass. A strong decrease in pH of the batch cultures due to ammonium consumption and CO₂ production by algae cells inhibited full glucose consumption for high concentrations of 17 g l⁻¹ glucose. Characterizing the amino acid content and pattern of the biomass revealed an amino acid fraction of 32.9% with Alanine as the most frequent amino acid.

这项研究调查了纤维素酶催化的报纸废料和纸浆作为碳源，再结合厌氧消化的消化液作为氮源，从而对小球藻进行了异养培养。纸浆的酶促水解产生了包含27.2 g l ^-1葡萄糖的水解产物，反映了78％的纤维素转化率。在消耗9.7g l ^-1葡萄糖的情况下，在培养91小时内达到了3.64 g l ^-1的小球藻最大生物量产量。最有效的培养将1 g l ^-1葡萄糖转化为0.42 g l ^-1生物质。由于高浓度的17 g l ^-1葡萄糖消耗的铵盐和藻类细胞产生的CO ₂导致分批培养物的pH值大大降低，从而抑制了全部葡萄糖的消耗。表征生物质的氨基酸含量和模式后，发现氨基酸含量为32.9％，其中丙氨酸为最常见的氨基酸。