Pulmonary toxoplasmosis is rare in immunocompetent patients. Herein, a Toxoplasma gondii strain isolated in Brazil from an immunocompetent patient who had severe pulmonary involvement was biologically and molecularly characterized for the first time. The TgHumIMTBr1 isolate was bioassayed in mice showing a virulent phenotype. Restriction fragment length polymorphism (RFLP) genotyping using 11 markers [SAG1, SAG2 (5´3´SAG2 and alt. SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3] revealed a new non-archetypal genotype assigned as #312. Genotyping using ROP18/ROP5 markers exhibited the virulent combination of alleles 4 and 1. Microsatellite analysis using 15 markers (TUB2, W35, TgM-A, B18, B17, M33, IV.1, X1.1, N60, N82, AA, N61, N83, M48 and M102) revealed an atypical genotype with three unique alleles and a rare combination of alleles 246 (W35) and 203 (TgM-A) that is typical of the Amazon region. Non-archetypal genotypes with unique alleles may function in the occurrence of severe toxoplasmosis in immunocompetent patients in Brazil. Attempts to isolate or molecularly detect T. gondii for further genotyping studies would contribute to the understanding of causes related to the severity of toxoplasmosis in immunocompetent patients.

肺弓形虫病在免疫功能正常的患者中很少见。在这里，弓形虫在巴西，从一名具有免疫能力的严重肺部感染患者中分离出的菌株首次进行了生物学和分子鉴定。对TgHumIMTBr1分离株进行了生物测定，显示出有力的表型。显示了使用11个标记的限制性片段长度多态性（RFLP）基因型[SAG1，SAG2（5´3´SAG2和alt.SAG2），SAG3，BTUB，GRA6，c22-8，c29-2，L358，PK1，Apico和CS3]一个新的非原型基因型，分配为＃312。使用ROP18 / ROP5标记进行基因分型显示等位基因4和1的强毒组合。使用15个标记（TUB2，W35，TgM-A，B18，B17，M33，IV.1，X1.1，N60，N82，AA， N61，N83，M48和M102）显示了一个非典型基因型，具有三个独特的等位基因，以及罕见的等位基因246（W35）和203（TgM-A）组合，这是亚马逊地区的典型特征。具有独特等位基因的非原型基因型可能在巴西具有免疫能力的患者中发生严重弓形虫病时起作用。试图分离或分子检测刚地弓形虫的进一步基因分型研究将有助于理解与免疫能力强的患者弓形体病的严重程度有关的原因。