As a consequence of the preference for homologous tissues, bone banks are the primary source of bone and tendon grafts. However, the bacterial, viral, and fungal contamination of these grafts remains a considerable challenge in bone banks and often results in high rates of graft discarding and infections in patients. This study intended to investigate bacterial contamination in 509 bone grafts harvested from 110 multiorgan donors. Specimen collection included bone and soft tissue retrieved from culture-swabbing as well as bone and capsule for histopathology. Microbiological, histopathological, and radiographic analyses were carried out. Secondary sterilization was also conducted using cobalt 60 at the dose of 2.5 × 10⁴ Gy. There were 106 multi-organ donors. Of the 506 grafts, there were 54 Hemi pelvis, 191 femur, 142 tibia, and 119 fibulae. The surface swab contamination rate for all the grafts retrieved was 16.6%, and bone culture from all the grafts was 6.1%. When we looked at the incidence of contamination according to the location than the surface swab contamination rate for hemipelvis was 18 (33.3%), femur 30 (15.7%), tibia 21(14.7%) and fibula 15 (12.6%). The bone cultures were hemipelvis 12 (22.2%) femur 8 (4.1%), tibia 5 (3.5%) and fibula 6 (5.04%). These findings suggest that separate harvesting of the grafts in reverse order may help prevent contamination. The study also recommends discarding all grafts contaminated even with low pathogenicity organisms. However, bioburden needs to be further investigated to be detected and reduced.

由于偏好同源组织，骨库是骨和肌腱移植物的主要来源。然而，这些移植物的细菌、病毒和真菌污染在骨库中仍然是一个相当大的挑战，并且经常导致移植物丢弃和患者感染的高比率。本研究旨在调查从 110 名多器官供体采集的 509 块骨移植物中的细菌污染。标本收集包括从培养拭子中取出的骨骼和软组织以及用于组织病理学的骨骼和胶囊。进行了微生物学、组织病理学和放射学分析。还使用剂量为 2.5 × 10 ^{4 的}钴 60 进行二次灭菌 吉。多器官捐献者106人。在 506 个移植物中，有 54 个半骨盆、191 个股骨、142 个胫骨和 119 个腓骨。取回的所有移植物的表面拭子污染率为 16.6%，所有移植物的骨培养率为 6.1%。当我们根据位置查看污染发生率时，半骨盆的表面拭子污染率为 18 (33.3%)、股骨 30 (15.7%)、胫骨 21 (14.7%) 和腓骨 15 (12.6%)。骨培养物为半骨盆 12 (22.2%) 股骨 8 (4.1%)、胫骨 5 (3.5%) 和腓骨 6 (5.04%)。这些发现表明，以相反的顺序单独收获移植物可能有助于防止污染。该研究还建议丢弃所有被污染的移植物，即使是低致病性生物。然而，生物负载需要进一步研究以检测和减少。