Microbial communities from a lake and river flowing through a highly dense urbanized township in Malaysia were profiled by sequencing amplicons of the 16S V3–V4 and 18S V9 hypervariable rRNA gene regions via Illumina MiSeq. Results revealed that Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were the dominant prokaryotic phyla; whereas, eukaryotic communities were predominantly of the SAR clade and Opisthokonta. The abundance of Pseudomonas and Flavobacterium in all sites suggested the possible presence of pathogens in the urban water systems, supported by the most probable number (MPN) values of more than 1600 per 100 mL. Urbanization could have impacted the microbial communities as transient communities (clinical, water-borne and opportunistic pathogens) coexisted with common indigenous aquatic communities (Cyanobacteria). It was concluded that in urban water systems, microbial communities vary in their abundance of microbial phyla detected along the water systems. The influences of urban land use and anthropogenic activities influenced the physicochemical properties and the microbial dynamics in the water systems.

通过 Illumina MiSeq 对 16S V3-V4 和 18S V9 高变 rRNA 基因区域的扩增子进行测序，对流经马来西亚高度密集城市化城镇的湖泊和河流中的微生物群落进行了分析。结果表明，变形菌门、拟杆菌门、放线菌门和厚壁菌门是主要的原核生物门；而真核生物群落主要是 SAR 分支和 Opisthokonta。所有地点的假单胞菌和黄杆菌丰度表明城市水系统中可能存在病原体，每 100 毫升超过 1600 个的最可能数 (MPN) 值也支持这一点。城市化可能会影响微生物群落，因为短暂群落（临床、水传播和机会性病原体）与常见的本土水生群落（蓝藻）共存。结论是，在城市水系统中，沿水系统检测到的微生物群落的微生物门丰度各不相同。城市土地利用和人类活动的影响影响了水系统的理化性质和微生物动态。