Amomum tsao-ko (Zingiberaceae), an important traditional medicinal herb, possesses many biological activities, including anti-inflammatory effects. Though the anti-obesity properties of the crude ethanol extract of A. tsao-ko fruits have been reported, the anti-adipogenic properties of its phytochemical constituents have not been reported. Therefore, in the present study, we isolated the active constituents of A. tsao-ko and investigated their anti-adipogenic effects. The bioassay-guided isolation of the phytochemicals from the ethanol extract of A. tsao-ko fruits identified four bioactive compounds, comprising one fatty acid (1), one sesquiterpene alcohol (2), and two phenolic compounds (3 and 4). Their structures were elucidated by a combination of 1D and/or 2D nuclear magnetic resonance and mass spectrometry. The anti-adipogenic activities of the four compounds evaluated by Oil Red O staining in 3T3-L1 cells revealed that the treatment with the isolated compounds 1 and 3 reduced the lipid accumulation in 3T3-L1 adipocytes more strongly than the compounds 2 and 4, in a dose-dependent manner.

中文翻译：

---

生物测定指导下砂仁砂仁果实中肥胖植物化学成分的分离与鉴定

重要的传统药草砂仁（姜科）具有许多生物活性，包括抗炎作用。尽管已经报道了太子参乙醇粗提物的抗肥胖特性，但尚未报道其植物化学成分的抗脂肪形成特性。因此，在本研究中，我们分离了曹操的活性成分并研究了它们的抗脂肪形成作用。用生物测定指导的方法从拟南芥果实的乙醇提取物中分离出植物化学物质，鉴定出四种生物活性化合物，包括一种脂肪酸（1），一种倍半萜烯醇（2）和两种酚类化合物（3和4）。通过1D和/或2D核磁共振和质谱的结合阐明了它们的结构。