Background: Method dependent variation of caeruloplasmin measurement is significant and necessitates the requirement for assay-specific reference intervals. Local determination becomes necessary in the absence of suitable published or manufacturer quoted reference intervals.

Methods: Applicability of the Beckman Coulter AU quoted reference interval was determined by assay of 20 surplus serum samples from patients attending the medical renal stones clinic at University Hospital Southampton. Subsequently 60 additional samples were collected for local reference interval determination. Samples were analysed for caeruloplasmin using the Beckman Coulter AU turbidometric kit on an AU680 analyser. Outliers were removed and non-parametric rank analysis of the results was performed.

Results: The Beckman Coulter quoted reference interval of 200-600 mg/L was unsuitable with 40% of the verification samples falling below 200 mg/L. A caeruloplasmin reference interval of 150-320 mg/L was established.

Conclusion: Users should be aware the quoted Beckman Coulter AU turbidometric reference interval may not be appropriate. We have established a method-specific adult reference interval for routine use.

背景：血浆铜蓝蛋白的测定方法差异很大，因此需要特定于测定的参考间隔。在没有合适的发布或制造商引用参考间隔的情况下，必须进行本地确定。

方法：通过分析来自南安普敦大学附属医院肾结石诊所就诊患者的20份多余血清样本，确定Beckman Coulter AU引用参考区间的适用性。随后，收集了60个其他样本用于本地参考间隔测定。使用AU680分析仪上的Beckman Coulter AU浊度试剂盒分析样品中的铜蓝蛋白。除去异常值，并对结果进行非参数秩分析。

结果：Beckman Coulter引用的参考间隔为200-600 mg / L不适合，其中40％的验证样品降至200 mg / L以下。确立了caeruloplasmin参考间隔为150-320 mg / L。

结论：用户应注意，引用的Beckman Coulter AU浊度参考间隔可能不合适。我们为常规使用建立了特定于方法的成人参考间隔。