Olive quick decline syndrome (OQDS) is a multifactorial disease affecting olive plants. The onset of this economically devastating disease has been associated with a Gram-negative plant pathogen called Xylella fastidiosa (Xf). Liquid chromatography separation coupled to high-resolution mass spectrometry detection is one the most widely applied technologies in metabolomics, as it provides a blend of rapid, sensitive, and selective qualitative and quantitative analyses with the ability to identify metabolites. The purpose of this work is the development of a global metabolomics mass spectrometry assay able to identify OQDS molecular markers that could discriminate between healthy (HP) and infected (OP) olive tree leaves. Results obtained via multivariate analysis through an HPLC-ESI HRMS platform (LTQ-Orbitrap from Thermo Scientific) show a clear separation between HP and OP samples. Among the differentially expressed metabolites, 18 different organic compounds highly expressed in the OP group were annotated; results obtained by this metabolomic approach could be used as a fast and reliable method for the biochemical characterization of OQDS and to develop targeted MS approaches for OQDS detection by foliage analysis.

中文翻译：

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HPLC-HRMS全球代谢组学方法诊断橄榄树叶片中的“橄榄快速下降综合征”标记

橄榄快速衰退综合症（OQDS）是影响橄榄植物的多因素疾病。这种经济上具有破坏性的疾病的发作与革兰氏阴性植物病原体Xylella fastidiosa有关（Xf）。液相色谱分离与高分辨率质谱检测相结合是代谢组学中应用最广泛的技术之一，因为它结合了快速，灵敏，选择性的定性和定量分析以及鉴定代谢物的能力。这项工作的目的是开发一种全球代谢组学质谱分析方法，该方法能够鉴定可区分健康（HP）和受感染（OP）橄榄树叶子的OQDS分子标记。通过HPLC-ESI HRMS平台（Thermo Scientific的LTQ-Orbitrap）通过多变量分析获得的结果表明，HP和OP样品之间有明显的分离。在差异表达的代谢产物中，标注了OP组中高表达的18种不同的有机化合物。