Mitochondria are devoid of poly(ADP‐ribose)polymerase‐1, but harbor its product oligo(ADP‐ribose),Journal of Cellular Biochemistry

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Mitochondria are devoid of poly(ADP‐ribose)polymerase‐1, but harbor its product oligo(ADP‐ribose)
Journal of Cellular Biochemistry ( IF 3.0 ) Pub Date : 2021-01-08 , DOI: 10.1002/jcb.29887
Julia Köritzer ₁ , Christian Blenn ₂ , Alexander Bürkle ₁ , Sascha Beneke _{1,

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Affiliation

There are conflicting data about localization of poly(ADP‐ribose)polymerase‐1 and its product poly(ADP‐ribose) in mitochondria. To finally clarify the discussion, we investigated with biochemical and cell biological methods the potential presence of poly(ADP‐ribose) polymerase‐1 in these organelles. Our data show that endogenous and overexpressed poly(ADP‐ribose)polymerase 1 is only localized to the nucleus with a clear exclusion of cytosolic compartments. In addition, highly purified mitochondria devoid of nuclear contaminations do not contain poly(ADP‐ribose)polymerase‐1. Although no poly(ADP‐ribose)polymerase‐1 enzyme is detectable in mitochondria, a shorter variant of its product poly(ADP‐ribose) is present, associated specifically with a small subset of mitochondrial proteins as revealed by immunoprecipitation and protein fingerprint analysis. These proteins are located at key‐points of the Krebs‐cycle, are chaperones involved in mitochondrial functionality and quality‐control, and are RNA‐binding proteins important for transcript stability, respectively. Of note, despite the fact that especially poly(ADP‐ribose)polymerase‐1 is its own major target for modification, we could not detect this enzyme by mass spectrometry in these organelles. These data suggests a new way of targeted nuclear‐mitochondrial signaling, mediated by nuclear poly(ADP‐ribosyl)ation dependent on poly(ADP‐ribose)polymerase‐1.

中文翻译：

线粒体缺乏聚（ADP-核糖）聚合酶-1，但含有其产物寡聚（ADP-核糖）

关于聚（ADP-核糖）聚合酶-1 及其产物聚（ADP-核糖）在线粒体中的定位存在相互矛盾的数据。为了最终澄清讨论，我们用生化和细胞生物学方法研究了这些细胞器中聚（ADP-核糖）聚合酶-1 的潜在存在。我们的数据表明，内源性和过度表达的聚（ADP-核糖）聚合酶 1 仅定位于细胞核，并明显排除了胞质区室。此外，没有核污染的高度纯化的线粒体不含聚（ADP-核糖）聚合酶-1。尽管在线粒体中检测不到聚（ADP-核糖）聚合酶-1 酶，但存在其产物聚（ADP-核糖）的较短变体，通过免疫沉淀和蛋白质指纹分析显示，它与一小部分线粒体蛋白特异性相关。这些蛋白质位于三羧酸循环的关键点，是参与线粒体功能和质量控制的分子伴侣，并且分别是对转录物稳定性很重要的 RNA 结合蛋白。值得注意的是，尽管聚（ADP-核糖）聚合酶-1 是其自身的主要修饰目标，但我们无法通过质谱法在这些细胞器中检测到这种酶。这些数据表明了一种新的靶向核线粒体信号传导方式，由依赖于聚（ADP-核糖）聚合酶-1 的核聚（ADP-核糖基）化介导。尽管事实上聚（ADP-核糖）聚合酶-1 是其自身的主要修饰目标，但我们无法通过质谱法在这些细胞器中检测到这种酶。这些数据表明了一种新的靶向核线粒体信号传导方式，由依赖于聚（ADP-核糖）聚合酶-1 的核聚（ADP-核糖基）化介导。尽管事实上聚（ADP-核糖）聚合酶-1 是其自身的主要修饰目标，但我们无法通过质谱法在这些细胞器中检测到这种酶。这些数据表明了一种新的靶向核线粒体信号传导方式，由依赖于聚（ADP-核糖）聚合酶-1 的核聚（ADP-核糖基）化介导。

更新日期：2021-01-08

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