Spatial distribution of mechanical properties in Pseudomonas aeruginosa biofilms, and their potential impacts on biofilm deformation,Biotechnology and Bioengineering

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Spatial distribution of mechanical properties in Pseudomonas aeruginosa biofilms, and their potential impacts on biofilm deformation
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2021-01-07 , DOI: 10.1002/bit.27671
Juan P Pavissich _{1,

2,

3} , Mengfei Li ₃ , Robert Nerenberg ₃

Affiliation

The mechanical properties of biofilms can be used to predict biofilm deformation under external forces, for example, under fluid flow. We used magnetic tweezers to spatially map the compliance of Pseudomonas aeruginosa biofilms at the microscale, then applied modeling to assess its effects on biofilm deformation. Biofilms were grown in capillary flow cells with Reynolds numbers (Re) ranging from 0.28 to 13.9, bulk dissolved oxygen (DO) concentrations from 1 mg/L to 8 mg/L, and bulk calcium ion (Ca²⁺) concentrations of 0 and 100 mg CaCl₂/L. Higher Re numbers resulted in more uniform biofilm morphologies. The biofilm was stiffer at the center of the flow cell than near the walls. Lower bulk DO led to more stratified biofilms. Higher Ca²⁺ concentrations led to increased stiffness and more uniform mechanical properties. Using the experimental mechanical properties, fluid–structure interaction models predicted up to 64% greater deformation for heterogeneous biofilms, compared with a homogeneous biofilms with the same average properties. However, the deviation depended on the biofilm morphology and flow regime. Our results show significant spatial mechanical variability exists at the microscale, and that this variability can potentially affect biofilm deformation. The average biofilm mechanical properties, provided in many studies, should be used with caution when predicting biofilm deformation.

中文翻译：

铜绿假单胞菌生物膜力学性能的空间分布及其对生物膜变形的潜在影响

生物膜的机械性能可用于预测生物膜在外力作用下的变形，例如在流体流动下。我们使用磁性镊子在空间上绘制铜绿假单胞菌生物膜在微观尺度上的顺应性，然后应用建模来评估其对生物膜变形的影响。生物膜在雷诺数 (Re) 范围从 0.28 到 13.9、散装溶解氧 (DO) 浓度从 1 mg/L 到 8 mg/L 和散装钙离子 (Ca ²⁺ ) 浓度为 0 和100 毫克 CaCl ₂ /L。较高的 Re 数导致更均匀的生物膜形态。流动池中心的生物膜比壁附近的生物膜更硬。较低的体积 DO 导致更多分层的生物膜。高钙²⁺浓度导致增加的刚度和更均匀的机械性能。使用实验机械性能，流固耦合模型预测，与具有相同平均性能的均质生物膜相比，异质生物膜的变形量最多可增加 64%。然而，偏差取决于生物膜形态和流动状态。我们的结果表明，在微观尺度上存在显着的空间机械变异性，并且这种变异性可能会影响生物膜变形。在预测生物膜变形时，应谨慎使用许多研究中提供的平均生物膜机械性能。

更新日期：2021-03-17

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