Inflammation is a significant clinical problem that can arise from full-thickness wounds or burn injuries or microbial disease. Although topical wound healing substances could promote rapid wound healing by preventing or reducing the consequences of inflammation, there still remains a need for the development of novel substances that can effectively reduce infection and inflammation in initial wound healing phase. In this study, collagen was combined with asiaticoside (AS) and ε-poly-l-lysine (εPLL). This complex was then applied to in vitro models of infection and inflammation. Collagen-AS coatings inhibited the initial inflammatory response to LPS through a sustained release of AS, and a bilayer coating-εPLL showed a notable antimicrobial effect using microbial infection test. In this study, we determined whether asiaticoside and εPLL have anti-inflammatory and antibacterial effects through different mechanisms. Collectively, the collagen-AS/εPLL complex indicated great therapeutic potentials for accelerate wound healing and the complex may be considered as a artificial scaffold substitute product to full-thickness wound healing.

炎症是由全层伤口或烧伤或微生物疾病引起的重要临床问题。尽管局部伤口愈合物质可以通过预防或减少炎症的后果来促进伤口的快速愈合，但是仍然需要开发可以在初期伤口愈合阶段有效减少感染和炎症的新型物质。在这项研究中，胶原蛋白与积雪草苷（AS）和ε-聚-1-赖氨酸（εPLL）结合使用。然后将该复合物应用于体外感染和炎症的模型。胶原蛋白AS涂层通过AS的持续释放抑制了对LPS的初始炎症反应，双层涂层εPLL使用微生物感染测试显示出显着的抗菌作用。在这项研究中，我们确定了积雪草苷和εPLL是否通过不同的机制具有抗炎和抗菌作用。总的来说，胶原-AS /εPLL复合物显示出加速伤口愈合的巨大治疗潜力，并且该复合物可以被认为是全厚度伤口愈合的人工支架替代产品。