In this study, electrospun scaffolds were fabricated by blending poly(l-lactide-co-ε-caprolactone) (PLCL) and silk fibroin (SF) with different ratios, and further the feasibility of electrospun PLCL/SF scaffolds were evaluated for application of tissue engineered heart valve (TEHV). Scanning electron microscopy (SEM) results showed that the surface of PLCL/SF electrospun scaffolds was smooth and uniform while the mechanical properties were appropriate as valve prosthesis. In vitro cytocompatibility evaluation results demonstrated that all of the PLCL/SF electrospun scaffolds were cytocompatible and valvular interstitial cells (VICs) cultured on PLCL/SF scaffolds of 80/20 & 70/30 ratios exhibited the best cytocompatibility. The in vitro osteogenic differentiation of VICs including alkaline phosphatase (ALP) activity and quantitative polymerase chain reaction (qPCR) assays indicated that PLCL/SF scaffolds of 80/20 & 90/10 ratios behaved better anti-calcification ability. In the in vivo calcification evaluation model of rat subdermal implantation, PLCL/SF scaffolds of 80/20 & 90/10 ratios presented better anti-calcification ability, which was consistent with the in vitro results. Moreover, PLCL/SF scaffolds of 80/20 & 70/30 ratios showed significantly enhanced cell infiltration and M2 macrophage with higher CD206+/CD68+ ratio. Collectively, our data demonstrated that electrospun scaffolds with the PLCL/SF ratio of 80/20 hold great potential as TEHV materials.

在本研究中，通过将聚（l-丙交酯-co -ε-己内酯）（ PLCL）和丝素蛋白（SF）以不同比例混合制备电纺支架，并进一步评估了电纺PLCL / SF支架在应用中的可行性。组织工程心脏瓣膜（TEHV）。扫描电镜（SEM）结果表明，PLCL/SF静电纺丝支架表面光滑、均匀，力学性能与瓣膜假体相适应。体外细胞相容性评估结果表明，所有 PLCL/SF 电纺支架均具有细胞相容性，并且在 80/20 和 70/30 比例的 PLCL/SF 支架上培养的瓣膜间质细胞 (VIC) 表现出最佳的细胞相容性。体外的VIC 的成骨分化包括碱性磷酸酶 (ALP) 活性和定量聚合酶链反应 (qPCR) 测定表明，比例为 80/20 和 90/10 的 PLCL/SF 支架具有更好的抗钙化能力。在大鼠皮下植入体内钙化评价模型中，80/20和90/10比例的PLCL/SF支架具有更好的抗钙化能力，与体外结果一致。此外，80/20 和 70/30 比例的 PLCL/SF 支架显示出显着增强的细胞浸润和 M2 巨噬细胞，具有更高的 CD206+/CD68+ 比例。总的来说，我们的数据表明，PLCL/SF 比为 80/20 的静电纺丝支架作为 TEHV 材料具有巨大的潜力。