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Biofabrication of poly(l-lactide-co-ε-caprolactone)/silk fibroin scaffold for the application as superb anti-calcification tissue engineered prosthetic valve
Biomaterials Advances ( IF 5.5 ) Pub Date : 2021-01-08 , DOI: 10.1016/j.msec.2021.111872
Xiaoxiao Wang 1 , Jing Liu 2 , Huimin Jing 1 , Binhan Li 1 , Zhiting Sun 1 , Boxuan Li 1 , Deling Kong 3 , Xigang Leng 1 , Zhihong Wang 1
Affiliation  

In this study, electrospun scaffolds were fabricated by blending poly(l-lactide-co-ε-caprolactone) (PLCL) and silk fibroin (SF) with different ratios, and further the feasibility of electrospun PLCL/SF scaffolds were evaluated for application of tissue engineered heart valve (TEHV). Scanning electron microscopy (SEM) results showed that the surface of PLCL/SF electrospun scaffolds was smooth and uniform while the mechanical properties were appropriate as valve prosthesis. In vitro cytocompatibility evaluation results demonstrated that all of the PLCL/SF electrospun scaffolds were cytocompatible and valvular interstitial cells (VICs) cultured on PLCL/SF scaffolds of 80/20 & 70/30 ratios exhibited the best cytocompatibility. The in vitro osteogenic differentiation of VICs including alkaline phosphatase (ALP) activity and quantitative polymerase chain reaction (qPCR) assays indicated that PLCL/SF scaffolds of 80/20 & 90/10 ratios behaved better anti-calcification ability. In the in vivo calcification evaluation model of rat subdermal implantation, PLCL/SF scaffolds of 80/20 & 90/10 ratios presented better anti-calcification ability, which was consistent with the in vitro results. Moreover, PLCL/SF scaffolds of 80/20 & 70/30 ratios showed significantly enhanced cell infiltration and M2 macrophage with higher CD206+/CD68+ ratio. Collectively, our data demonstrated that electrospun scaffolds with the PLCL/SF ratio of 80/20 hold great potential as TEHV materials.



中文翻译:

聚(l-丙交酯-co-ε-己内酯)/丝素蛋白支架的生物制造,可用作极好的抗钙化组织工程人工瓣膜

在本研究中,通过将聚(l-丙交酯-co -ε-己内酯)( PLCL)和丝素蛋白(SF)以不同比例混合制备电纺支架,并进一步评估了电纺PLCL / SF支架在应用中的可行性。组织工程心脏瓣膜(TEHV)。扫描电镜(SEM)结果表明,PLCL/SF静电纺丝支架表面光滑、均匀,力学性能与瓣膜假体相适应。体外细胞相容性评估结果表明,所有 PLCL/SF 电纺支架均具有细胞相容性,并且在 80/20 和 70/30 比例的 PLCL/SF 支架上培养的瓣膜间质细胞 (VIC) 表现出最佳的细胞相容性。体外VIC 的成骨分化包括碱性磷酸酶 (ALP) 活性和定量聚合酶链反应 (qPCR) 测定表明,比例为 80/20 和 90/10 的 PLCL/SF 支架具有更好的抗钙化能力。在大鼠皮下植入体内钙化评价模型中,80/20和90/10比例的PLCL/SF支架具有更好的抗钙化能力,与体外结果一致。此外,80/20 和 70/30 比例的 PLCL/SF 支架显示出显着增强的细胞浸润和 M2 巨噬细胞,具有更高的 CD206+/CD68+ 比例。总的来说,我们的数据表明,PLCL/SF 比为 80/20 的静电纺丝支架作为 TEHV 材料具有巨大的潜力。

更新日期:2021-01-18
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