The aim of this work was to investigate the photocatalytic inactivation efficiencies of eight phages infective to lactic acid bacteria (LAB) contained in bioaerosols. Studies were performed in a semi-pilot scale equipment, which consists of a stainless steel camera, in which air containing the phage particles circulates, and an acrylic reactor with twenty UV-A lamps and six borosilicate plates covered with the catalytic film (TiO₂). Phages (ALQ13.2, ATCC 15807-B1, ATCC 8014-B1, BYM, CHD, LDG, Ln-9 and MLC-A) were nebulized into the camera using the 6-jet Collison nebulizer, being assays performed for a total time of 100 min. Sampling by impact of air on a glass slide was carried out. Some conditions of equipment operation (phage suspension concentration, nebulization method, influence of relative humidity) using phage ATCC 8014-B1 were previously adjusted. Phages ALQ13.2, ATCC 15807-B1, BYM and CHD were not detected in the air because of loss of their infectivity after nebulization when assays in absence of catalyst and UV radiation were conducted. On the other hand, it was possible to achieve a complete inactivation (< 10 PFU mL⁻¹) of phages ATCC 8014-B1, LDG and Ln-9 within 100 min of photocatalytic treatments. On the other, phage MLC-A was partially inactivated under the same conditions. These results are in agreement to those obtained when photonic and quantum inactivation efficiencies were calculated. In this sense, efficiencies varying between 1.27–2.64 × 10⁻¹⁷ PFU photon⁻¹ and 4.42–9.18 × 10⁻¹⁷ PFU photon⁻¹ were obtained, respectively, being the lowest efficiencies for phage MLC-A. This phage with the highest resistance could be studied in the future to evaluate diverse working conditions in order to optimize the operation and efficiency of the semi-pilot scale equipment.

这项工作的目的是研究八种感染生物气溶胶中乳酸菌（LAB）的噬菌体的光催化灭活效率。研究是在半试验规模的设备中进行的，该设备由不锈钢相机组成，在该相机中流通有噬菌体颗粒的空气在其中流通；丙烯酸反应器配有20个UV-A灯和6个覆盖有催化膜（TiO _2的硼硅酸盐板））。使用6喷嘴Collison雾化器将噬菌体（ALQ13.2，ATCC 15807-B1，ATCC 8014-B1，BYM，CHD，LDG，Ln-9和MLC-A）雾化到相机中，进行总时间的分析100分钟 通过在玻璃载片上的空气冲击进行采样。预先调整了使用噬菌体ATCC 8014-B1进行设备操作的一些条件（噬菌体悬浮液浓度，雾化方法，相对湿度的影响）。在无催化剂和无紫外线辐射的条件下进行雾化后，由于在雾化后它们的感染力丧失，因此未在空气中检测到噬菌体ALQ13.2，ATCC 15807-B1，BYM和CHD。另一方面，有可能实现完全灭活（<10 PFU mL ^-1）在100分钟的光催化处理后噬菌体ATCC 8014-B1，LDG和Ln-9。另一方面，噬菌体MLC-A在相同条件下被部分灭活。这些结果与计算光子和量子灭活效率时获得的结果一致。从这个意义上讲，获得的效率分别在1.27–2.64×10 ^-17 PFU光子^-1和4.42–9.18×10 ^-17 PFU光子^-1之间，这是噬菌体MLC-A的最低效率。这种具有最高抗性的噬菌体可在未来进行研究，以评估各种工作条件，以优化半自动规模设备的操作和效率。