Trypsin is used to remove non-collagenous proteins from cattle hide in leather processing but prone to damage hide surface and reduce leather quality due to its rapid protein hydrolysis action on the hide surface and slow penetration through the whole hide. A novel trypsin bating method was developed using a reversible inhibition of trypsin with high-yield, low-cost and environment-friendly soybean flour (SF). When the mass ratio of trypsin to SF was 1:20, the trypsin activity was decreased by 77 % at a reaction time of 5 min and completely returned in 100 min because of the competitive Kunitz trypsin inhibitor in SF. Bating with trypsin-SF mixture avoided the damage to hide surface caused by trypsin, improved the dispersion uniformity of hide collagen fibers and increased the softness and the mechanical strength of leather. This work exhibits huge potential for improving the leather quality and provides new insights into the soybean application.

胰蛋白酶用于在皮革加工过程中从牛皮革中去除非胶原蛋白，但由于其在皮革表面上的快速蛋白质水解作用和在整个皮革中的缓慢渗透作用，容易损坏皮革表面并降低皮革质量。开发了一种新颖的胰蛋白酶消化方法，该方法利用高产量，低成本和环境友好的大豆粉（SF）可逆抑制胰蛋白酶来进行。当胰蛋白酶与SF的质量比为1:20时，由于SF中具有竞争性的Kunitz胰蛋白酶抑制剂，胰蛋白酶活性在5分钟的反应时间内降低了77％，并在100分钟内完全恢复。用胰蛋白酶-SF混合物软化避免了胰蛋白酶对皮革表面的损害，改善了皮革胶原纤维的分散均匀性，并提高了皮革的柔软度和机械强度。