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Detection of Giardia and Cryptosporidium in environmental matrices with immunomagnetic separation: two or three acid dissociations
Parasitology Research ( IF 2 ) Pub Date : 2021-01-08 , DOI: 10.1007/s00436-020-06999-4
Fernando César Andreoli , Lyda Patricia Sabogal-Paz

This study evaluated the technology of detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in environmental matrices obtained after water treatment on a bench scale. Calcium carbonate flocculation with immunomagnetic separation was the selected method to quantify the protozoa, and the importance of the number of acid dissociations in the immunomagnetic separation was assessed. When adding the third acid dissociation, an increase of 71% ± 6 in floated residue and 31.9% ± 28.7 in filter backwash water in cyst recovery was observed, while in oocyst recovery, a non-significant increase was detected. In the filtered water, this increased dissociation was important in the protozoa recovery with increases greater than 33%. The results showed that there is a strong interaction of these target organisms with the magnetic microspheres, since protozoa were still recovered in the third acid dissociation and some of them were still adhered to the magnetic microspheres.



中文翻译:

免疫磁分离检测环境基质中的贾第鞭毛虫和隐孢子虫:两种或三种酸解离

本研究评估了贾第鞭毛虫的检测技术。囊肿和隐孢子虫spp。台式水处理后获得的环境基质中的卵囊。碳酸钙絮凝和免疫磁分离是定量原生动物的选择方法,并且评估了酸解离数在免疫磁分离中的重要性。当添加第三次酸离解时,在囊肿回收中观察到浮选残渣增加了71%±6%,在过滤器反洗水中增加了31.9%±28.7%,而在卵囊回收中则未发现明显增加。在过滤后的水中,这种离解的增加对原生动物的恢复很重要,增加的幅度大于33%。结果表明,这些目标生物与磁性微球有很强的相互作用,

更新日期:2021-01-08
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