The monogenean genus Heterobothrium (Monogenea: Diclidophoridae) currently consists of 12 accepted species described globally from various pufferfishes (Tetraodontidae). This includes the economically important Heterobothrium okamotoi Ogawa 1991 that causes severe disease in the cultured tiger puffer Takifugu rubripes in Japan. During parasitological surveys targeting near shore marine fishes of South Africa, a new Heterobothrium was discovered on the gills of five evileye pufferfish Amblyrhynchotes honckenii with a prevalence of 100% and mean intensity of 23 (4–72). Specimens were morphologically studied using both light and scanning electron microscopy and molecularly characterized by sequencing the partial ribosomal gene 28S. Heterobothrium victorwepeneri n. sp. differs from its 12 congeners by a combination of morphological characters, such as fourth clamp pair (anteriormost, 180° inverted) as the smallest with some of the sclerites differing in shape from the other pairs, 8 to 9 genital hooks, number of testes 40–50, and absent isthmus. The 28S tree depicted two main branches, one clustering together species of mazocraeidean families while the other clustered together only species of the Diclidophoridae. Heterobothrium victorwepeneri n. sp. is the first species of its genus to be recorded and described from South Africa and from the tetraodontid A. honckenii. This study also provides for the first time 28S sequence for a species of this highly host-specific genus.

单属异形He属（Monogenea：Diclidophoridae）目前由全球范围内从各种河豚（Tetraodontidae）描述的12个公认物种组成。这包括具有重要经济意义的冈本氏异形假丝酵母小川1991年，该病在日本养殖的老虎河豚（Takifugu rubripes）中引起严重疾病。在针对南非近岸海鱼的寄生虫调查中，在五只邪恶之眼河豚A（Amblyrhynchotes honckenii）的the上发现了一种新的异腐杆菌患病率为100％，平均强度为23（4–72）。使用光学显微镜和扫描电子显微镜对标本进行形态学研究，并通过对部分核糖体基因28S进行测序进行分子表征。异内victorwepeneri ñ。sp。通过形态特征的组合与它的12个同类物不同，例如第四对钳夹（最前面，倒置180°），其中一些巩膜的形状不同于其他钳夹，有8至9个生殖器钩，睾丸数量40 –50，并且没有峡部。28S树描绘了两个主要分支，一个分支聚集了马祖龟科的物种，而另一棵仅聚集了双歧科的物种。维克多氏杆菌。sp。是从南非和四齿类A. honckenii记录和描述的该属的第一个物种。这项研究还首次提供了这种高度宿主特异性属的28S序列。