Introduction

Intestinal microbiota and metabolites play important roles for further improvement of animal production. Metabolomics of shrimp intestine to understand roles and their relationship to the host is hampered by the lack of metabolome profiling method.

Objectives

This study aims to develop extraction and analytical methods to allow accurate metabolic analysis in shrimp intestine.

Methods

Conditions for extraction and LC-HRMS/MS analysis were optimized.

Results

Extraction with ethyl acetate:acetone (15:2 v/v) acidified with 0.5% acetic acid, elution with acetonitrile:water acidified with 0.01% acetic acid for 25 min, and mass fragmentation at 15% HCD were the optimal conditions, yielding the highest signal intensity and numbers of putative metabolites.

Conclusion

Our method enabled in-depth study for shrimp-microbial interaction at metabolite level.

中文翻译：

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使用 LC-HRMS/MS 优化虾肠代谢物提取和分析方法进行代谢组学分析

介绍

肠道微生物群和代谢物在进一步提高动物生产方面发挥着重要作用。缺乏代谢组分析方法阻碍了虾肠代谢组学了解作用及其与宿主的关系。

目标

本研究旨在开发提取和分析方法，以便对虾肠道进行准确的代谢分析。

方法

优化了萃取和 LC-HRMS/MS 分析的条件。

结果

用 0.5% 乙酸酸化的乙酸乙酯:丙酮 (15:2 v/v) 提取，用 0.01% 乙酸酸化的乙腈:水洗脱 25 分钟，15% HCD 质量碎裂是最佳条件，得到最高信号强度和推定代谢物的数量。

结论

我们的方法能够在代谢物水平上对虾-微生物相互作用进行深入研究。