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SH2 domain-containing protein tyrosine phosphatase-2 (SHP-2) prevents cardiac remodeling after myocardial infarction through ERK/SMAD signaling pathway
Human Cell ( IF 3.4 ) Pub Date : 2021-01-08 , DOI: 10.1007/s13577-020-00430-x
Yong-Gang Lu 1 , He Tan 1 , Qian Ma 1 , Xin-Xin Li 1 , Jia Cui 1 , Xue Zhang 2 , Xue-Lei Liang 2 , Yan-Qing Tie 1
Affiliation  

In this study, we aimed to investigate the role of SH2 domain-containing protein tyrosine phosphatase-2 (SHP-2) in cardiac remodeling after myocardial infarction (MI) and explore the underlying molecular mechanism. MI model was established by ligation of the left anterior descending coronary artery. C57/BL6J mice were randomly administered with 3.0 mg/kg/day PHPS1 (PHPS1-treated group) or normal saline (model group) by intraperitoneal injection. After 4 weeks of infusion, the effects of PHPS1 on cardiac remodeling were evaluated. Echocardiography results showed that PHPS1 treatment aggravated the MI-induced deterioration of cardiac function, with worse cardiac function parameters. PHPS1 treatment significantly increased the infarcted area, as well as the fibrotic area and the expression of collagen I and collagen III. Western blots and immunofluorescence staining showed that PHPS1 treatment up-regulated the expression of p-GRK2, p-SMAD2/3 and p-ERK1/2, while U0126 reversed the effect of PHPS1. The present study indicated that PHPS1 treatment contributed to myocardial fibrosis and infarction by activating ERK/SMAD signaling pathway, suggesting that SHP-2 may be a promising treatment target for cardiac remodeling after MI.



中文翻译:


含SH2结构域的蛋白酪氨酸磷酸酶2(SHP-2)通过ERK/SMAD信号通路预防心肌梗死后心脏重塑



在本研究中,我们旨在探讨含SH2结构域的蛋白酪氨酸磷酸酶2(SHP-2)在心肌梗死(MI)后心脏重塑中的作用,并探讨其潜在的分子机制。结扎左冠状动脉前降支建立MI模型。 C57/BL6J小鼠随机腹腔注射3.0 mg/kg/天PHPS1(PHPS1治疗组)或生理盐水(模型组)。输注4周后,评估PHPS1对心脏重塑的影响。超声心动图结果显示,PHPS1治疗加剧了MI引起的心功能恶化,心功能参数更差。 PHPS1治疗显着增加了梗塞面积、纤维化面积以及胶原蛋白I和胶原蛋白III的表达。蛋白质印迹和免疫荧光染色显示 PHPS1 处理上调了 p-GRK2、p-SMAD2/3 和 p-ERK1/2 的表达,而 U0126 逆转了 PHPS1 的作用。本研究表明PHPS1治疗通过激活ERK/SMAD信号通路导致心肌纤维化和梗死,这表明SHP-2可能是MI后心脏重构的有前景的治疗靶点。

更新日期:2021-01-08
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