The mechanisms through which maternal immunization can modulate offspring thymic maturation of lymphocytes are not fully understood. Here, we aimed to evaluate whether maternal OVA-immunization can inhibit the maturation of Th17 cells on offspring thymus. C57BL/6 females were immunized with OVA in Alum or Alum alone and mated with normal WT males. Offspring thymus was evaluated at three or 20 days of age. The demonstration that maternal OVA-immunization can inhibit offspring allergy development validated our experimental protocol. First, we observed that maternal OVA-immunization can inhibit the expression of RORγT and IL-17 molecules on immature T cells (CD4+CD8+) and TCD4 cells (CD4+CD8⁻) without interference on TCD8 cells (CD4⁻CD8⁺) on three-day-old offspring. A very similar effect could be observed on 20-day-old offspring. Additionally, a Th2 skewed profile could be found on the spleen of immunized pups from OVA-immunized mothers, but no influence was detected on offspring thymic Th1/Th2 profiles. Together, these data demonstrate that maternal immunization with an allergen can modulate offspring thymic maturation of Th17 cells without influencing Th1/Th2 patterns.

母体免疫调节后代淋巴细胞胸腺成熟的机制尚不完全清楚。在这里，我们的目的是评估母体 OVA 免疫是否可以抑制后代胸腺上 Th17 细胞的成熟。 C57BL/6 雌性用明矾中的 OVA 或单独的明矾进行免疫，并与正常 WT 雄性交配。子代胸腺在 3 天或 20 天时进行评估。母体 OVA 免疫可以抑制后代过敏发展的证明验证了我们的实验方案。首先，我们观察到母体OVA免疫可以抑制未成熟T细胞（CD4+CD8+）和TCD4细胞（CD4+CD8-）上RORγT和IL-17分子的表达，而不干扰TCD8细胞（CD4 ^- CD8 ⁺ ）上的RORγT和IL- ¹⁷分子的表达。三天大的后代。在 20 天大的后代身上也可以观察到非常相似的效果。此外，在来自 OVA 免疫母体的免疫幼崽的脾脏中可以发现 Th2 倾斜特征，但未检测到对后代胸腺 Th1/Th2 特征的影响。总之，这些数据表明，母体使用过敏原进行免疫可以调节后代 Th17 细胞的胸腺成熟，而不影响 Th1/Th2 模式。