Cell Biology and Toxicology ( IF 5.3 ) Pub Date : 2021-01-07 , DOI: 10.1007/s10565-020-09577-7 Divya Bijukumar 1 , Abhijith Segu 1 , Paul Chastain 1, 2 , Mathew T Mathew 1, 3
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The complexity of cobalt-chromium-molybdenum (CoCrMo) nanoparticles generated from the hip modular taper interfaces resulted in inconclusive outcomes on the level of toxicity in orthopedic patients. We used a hip simulator to generate physiologically relevant CoCrMo degradation products (DPs) to demonstrate the variation in the level of toxicity in neurons in comparison to processed degradation products (PDPs). The study outcomes indicate that DP induces a higher level of DNA damage in the form of double- and single-stranded DNA breaks and alkaline labile DNA adducts versus PDPs. The scientific advancements of this study are the following: (i) how DPs mimic more closely to the implant debris from hip implants in terms of bioactivity, (ii) how hip implant debris causes local and systemic issues, and (iii) methods to augment the biologic impact of implant debris. We discovered that DP is bioactive compared with PDP, and this should be considered in the toxicity evaluation related to implants.
Graphical abstract
中文翻译：
植入物衍生的 CoCrMo 合金纳米颗粒破坏神经元细胞中的 DNA 复制动力学
从髋关节模块化锥形界面产生的钴铬钼 (CoCrMo) 纳米颗粒的复杂性导致骨科患者的毒性水平没有定论。我们使用髋关节模拟器生成生理相关的 CoCrMo 降解产物 (DPs)，以证明与加工降解产物 (PDP) 相比，神经元毒性水平的变化。研究结果表明，与 PDP 相比，DP 以双链和单链 DNA 断裂和碱性不稳定 DNA 加合物的形式诱导更高水平的 DNA 损伤。本研究的科学进展如下：(i) DPs 如何在生物活性方面更接近髋关节植入物的植入物碎片，(ii) 髋关节植入物碎片如何导致局部和全身问题，(iii) 增加植入物碎片生物影响的方法。我们发现 DP 与 PDP 相比具有生物活性，在与植入物相关的毒性评估中应考虑这一点。
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