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Cryopreservation of pineapple shoot tips does not affect mineral contents of regenerated plants
Acta Physiologiae Plantarum ( IF 2.4 ) Pub Date : 2021-01-08 , DOI: 10.1007/s11738-020-03195-w
Ariel Villalobos-Olivera , Julia Martínez , Doris Escalante , Marcos Edel Martínez-Montero , Sershen , José Carlos Lorenzo

The production of pineapple, one of the most economically important tropical fruits globally, is increasingly being threatened by biotic and abiotic factors. Germplasm conservation of pineapple is essential for sustaining breeding programs, and in this regard, cryopreservation is thought to be the best long-term storage option. This short communication describes the effects of pineapple shoot tip cryopreservation on subsequent growth and tissue (stems, leaves and fruit mesocarps) mineral content (Al, B, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, Ni, P, S, Sr and Zn) of plants during in vitro, acclimatization and field cultivation. Plants generated from liquid nitrogen exposed and control (unexposed) shoot tips did not differ significantly in terms of fresh weight at 45 days of in vitro growth, at 45 days of acclimatization or at 20 months of field cultivation. Similarly, levels of selected minerals were also not modified by cryopreservation. To the best of our knowledge, this is the first report of the potential effects of pineapple explant cryopreservation on mineral levels during the subsequent in vitro and field growth.



中文翻译:

菠萝芽尖端的低温保存不会影响再生植物的矿物质含量

菠萝是全球经济上最重要的热带水果之一,其生产正日益受到生物和非生物因素的威胁。菠萝的种质保存对于维持育种计划至关重要,因此,冷冻保存被认为是最佳的长期保存选择。这段简短的交流描述了菠萝芽尖端低温保存对随后的生长和组织(茎,叶和果实中果皮)矿物质含量(Al,B,Ba,Ca,Cu,Fe,K,Mg,Mn,Na,Ni,P的影响) ,S,Sr和Zn)在体外,驯化和田间栽培中的作用。从液氮暴露和对照(未暴露)枝梢产生的植物在体外生长45天,适应45天或田间栽培20个月时的鲜重无显着差异。同样,冷冻保存也不会改变所选矿物质的含量。据我们所知,这是菠萝外植体冷冻保存在随后的体外和田间生长过程中对矿物质水平的潜在影响的首次报道。

更新日期:2021-01-08
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