Background/Aim: Viral infection often exacerbates proteinuria, which has been suggested to be due to antiviral responses of podocytes. We examined the effect of polyinosinic-polycytidylic acid (polyIC) on the expression of retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) in differentiated human podocytes in culture. Materials and Methods: The podocytes were treated with 2 ng/ml to 500 μg/ml of polyIC for 3 to 36 h, and also transfected with siRNA against RIG-I and MDA5. F-actin staining was performed to assess actin reorganization. Results: PolyIC induced the expression of RIG-I and MDA5 in dose- and time-dependent manner, accompanied with interferon-β (IFN-β) and interleukin-6 (IL-6) up-regulation and actin reorganization. Temporal knockdown of RIG-I by siRNA decreased IFN-β expression, while MDA5 siRNA inhibited IFN-β and IL-6 expression. Actin reorganization was attenuated by RIG-I and MDA5 knockdown. Conclusion: RIG-I and MDA5 may play a role in the antiviral responses of podocytes.

中文翻译：

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PolyIC 诱导视黄酸诱导基因 I 和黑色素瘤分化相关基因 5 并调节足细胞炎症

背景/目的：病毒感染通常会加剧蛋白尿，这被认为是由于足细胞的抗病毒反应。我们检测了聚肌苷-聚胞苷酸 (polyIC) 对培养的分化人类足细胞中视黄酸诱导基因-I (RIG-I) 和黑色素瘤分化相关基因 5 (MDA5) 表达的影响。材料和方法：足细胞用 2 ng/ml 至 500 μg/ml 的 polyIC 处理 3 至 36 小时，并用针对 RIG-I 和 MDA5 的 siRNA 转染。进行 F-肌动蛋白染色以评估肌动蛋白重组。结果：PolyIC 以剂量和时间依赖性方式诱导 RIG-I 和 MDA5 的表达，伴随着干扰素-β (IFN-β) 和白细胞介素-6 (IL-6) 上调和肌动蛋白重组。siRNA 对 RIG-I 的暂时敲低降低了 IFN-β 表达，而 MDA5 siRNA 抑制了 IFN-β 和 IL-6 的表达。肌动蛋白重组被 RIG-I 和 MDA5 敲低减弱。结论：RIG-I和MDA5可能在足细胞的抗病毒反应中发挥作用。