Background/Aim: In vivo studies on pathogenesis of type 2 diabetes mellitus (T2DM) have been reported, however, the relationship between insulin-receptor substrate 1 (IRS1) and the area of Langerhans’ islets was unknown. Therefore, a correlation between both parameters was assessed. Materials and Methods: Diabetic groups were fed with a high-fat diet (HFD) and injected with three different doses of streptozotocin, namely 25, 35 and 45 mg/kg, and compared to a control group after 9 weeks. Results: Administration of HFD/streptozotocin increased the level of fasting blood glucose but reduced the level of IRS1 and the area of Langerhans’ islets in diabetic groups. The coefficient of correlation between IRS1 and area of Langerhans’ islets was 0.259 (p=0.232). In addition, the coefficient of correlation for fasting blood glucose with the area of Langerhans’ islets and IRS1 was −0.520 (p=0.011) and −0.603 (p=0.002), respectively. Conclusion: The reduction of IRS1 was weakly correlated with the destruction of Langerhans’ islets, suggesting there is an intermediate step between both parameters.

中文翻译：

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2型糖尿病大鼠模型中胰岛素受体底物1与朗格汉斯岛的关系

背景/目的：关于 2 型糖尿病 (T2DM) 发病机制的体内研究已有报道，但胰岛素受体底物 1 (IRS1) 与朗格汉斯胰岛面积之间的关系尚不清楚。因此，评估了两个参数之间的相关性。材料与方法：糖尿病组给予高脂饮食（HFD）并注射三种不同剂量的链脲佐菌素，即25、35和45mg/kg，9周后与对照组进行比较。结果：在糖尿病组中，HFD/链脲佐菌素提高了空腹血糖水平，但降低了 IRS1 水平和朗格汉斯胰岛面积。IRS1 与朗格汉斯岛面积之间的相关系数为 0.259 (p=0.232)。此外，空腹血糖与朗格汉斯胰岛面积和 IRS1 的相关系数分别为 -0.520 (p=0.011) 和 -0.603 (p=0.002)。结论：IRS1 的减少与朗格汉斯胰岛的破坏呈弱相关，表明这两个参数之间存在中间步骤。