Aim: Minichromosome maintenance (MCM) proteins are involved in initiation of DNA replication and cell-cycle progression. Loss of MCM function results in genomic instability and causes carcinogenesis. Among MCM genes, the role and prognostic value of MCM6 expression in clear-cell renal cell carcinoma (ccRCC) has not been elucidated. Materials and Methods: We assessed the mRNA expression level of MCM6 using the Gene Expression Profiling Interactive Analysis database and investigated MCM6 protein expression by immunohistochemistry in 238 ccRCC cases. Results: High MCM6 expression was significantly associated with increasing tumor size, pT, stage, tumor necrosis, and metastasis. Furthermore, high MCM6 expression was significantly associated with shorter overall and disease-free survival, and was an independent unfavorable prognostic marker. Regarding patients with metastasis, high MCM6-expressing ccRCC conferred significantly shorter survival than for those with low expression. Conclusion: A high MCM6 expression level may be a promising biomarker to predict tumor progression, metastasis, and survival in patients with ccRCC.

中文翻译：

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MCM6 高表达作为透明细胞肾细胞癌的潜在预后标志物

目的：微染色体维持 (MCM) 蛋白参与 DNA 复制的启动和细胞周期进程。MCM功能的丧失导致基因组不稳定并导致致癌。在 MCM 基因中，MCM6 表达在透明细胞肾细胞癌 (ccRCC) 中的作用和预后价值尚未阐明。材料和方法：我们使用 Gene Expression Profiling Interactive Analysis 数据库评估了 MCM6 的 mRNA 表达水平，并通过免疫组织化学研究了 238 例 ccRCC 病例中的 MCM6 蛋白表达。结果：高 MCM6 表达与肿瘤大小、pT、分期、肿瘤坏死和转移显着相关。此外，高 MCM6 表达与较短的总生存期和无病生存期显着相关，并且是一个独立的不利预后标志物。对于转移患者，高 MCM6 表达 ccRCC 的生存期明显短于低表达患者。结论：高 MCM6 表达水平可能是预测 ccRCC 患者肿瘤进展、转移和生存的有希望的生物标志物。