Background/Aim: Chronic cerebral hypoperfusion affects early and mature neurons in the subventricular zone (SVZ) and cerebral cortex. Herein, we investigated the effects of insulin-like growth factor-1 (IGF-1), a neurogenesis-promoting agent, on neurons in these regions in periventricular leucomalacia (PVL) model rats. Materials and Methods: Following right carotid artery ligation, the rats were placed in a hypoxia chamber and injected with recombinant IGF-1 (0.1 and 1 μg/μl). Their brain sections were immunohistochemically analysed using anti-nestin and anti-NeuN antibodies. Results: The numbers of early-neuronal cells in the SVZ and mature neurons in the cerebral cortex were higher and lower, respectively, in the PVL group than in the control group. The number of NeuN-positive cells was significantly higher in the IGF-treated group than in the PVL group. Conclusion: PVL increased the number of early neuronal cells in the SVZ, reducing the survival of mature neurons in the cerebral cortex; IGF-1 reversed these effects.

中文翻译：

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IGF-1 在脑室周围白质软化模型中保护皮层和脑室下区的神经元

背景/目的：慢性脑灌注不足会影响脑室下区 (SVZ) 和大脑皮层的早期和成熟神经元。在这里，我们研究了胰岛素样生长因子-1 (IGF-1)，一种神经发生促进剂，对脑室周围白质软化 (PVL) 模型大鼠这些区域的神经元的影响。材料与方法：结扎右颈动脉后，将大鼠置于缺氧室中，注射重组IGF-1（0.1和1 μg/μl）。使用抗巢蛋白和抗 NeuN 抗体对他们的脑切片进行免疫组织化学分析。结果：PVL组SVZ早期神经元细胞数量和大脑皮层成熟神经元数量分别高于对照组和低于对照组。IGF 处理组的 NeuN 阳性细胞数量显着高于 PVL 组。结论：PVL增加了SVZ早期神经元细胞的数量，降低了大脑皮层成熟神经元的存活率；IGF-1 逆转了这些影响。