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MicroRNA Deregulation in Papillary Thyroid Cancer and its Relationship With BRAF V600E Mutation
In Vivo ( IF 1.8 ) Pub Date : 2021-01-01 , DOI: 10.21873/invivo.12262
Petr Celakovsky 1 , Helena Kovarikova 2 , Viktor Chrobok 3 , Jan Mejzlik 3 , Jan Laco 4 , Hana Vosmikova 4 , Marcela Chmelarova 2 , Ales Ryska 4
Affiliation  

Background: MicroRNAs (miRNAs) are non-coding regulatory molecules 18-25 nucleotides in length that act as post-transcriptional regulators of gene expression. MiRNAs affect various biological processes including carcinogenesis. Deregulation of miRNAa expression has been described in a variety of tumors including papillary thyroid carcinoma (PTC). The aim of the present study was to investigate the role of selected miRNAs in PTC and find associations between miRNA expression and the BRAF (V600E) mutation. Materials and Methods: The study group comprised a total of 62 patients with surgically treated PTC. The control group consisted of 30 patients with nodular goitre that were surgically treated in the same time period. The expression status of miR-146b, miR-181a, miR-187, miR-221 and miR-222 was determined using quantitative real-time PCR. BRAF mutation analysis was performed by PCR with reverse hybridization. Results: MiR-146b, miR-181a, miR-187, miR-221 and miR-222 were up-regulated in PTC compared to normal thyroid gland tissue of the same patient. MiR-146b, miR-187, miR-221 and miR-222 were also up-regulated in PTC compared to nodular goitre. The recurrent tumors were statistically significantly associated with up-regulation of miR-221. The mutation V600E of BRAF gene was significantly associated with up-regulation of miR-146b and with down-regulation of miR-187. Conclusion: Over-expression of selected miRNAs in PTC compared to normal thyroid gland tissue and nodular goitre was found. Moreover, miR-221 may serve as a prognostic marker as its over-expression was significantly associated with recurrent tumors.

中文翻译:

甲状腺乳头状癌中的 microRNA 失调及其与 BRAF V600E 突变的关系

背景:MicroRNA (miRNA) 是长度为 18-25 个核苷酸的非编码调节分子,可作为基因表达的转录后调节因子。miRNA影响包括致癌作用在内的各种生物过程。已在包括甲状腺乳头状癌 (PTC) 在内的多种肿瘤中描述了 miRNAa 表达的失调。本研究的目的是调查选定的 miRNA 在 PTC 中的作用,并发现 miRNA 表达与 BRAF (V600E) 突变之间的关联。材料和方法:研究组共有 62 名接受手术治疗的 PTC 患者。对照组为同期接受手术治疗的30例结节性甲状腺肿患者。使用定量实时 PCR 确定 miR-146b、miR-181a、miR-187、miR-221 和 miR-222 的表达状态。BRAF 突变分析通过反向杂交的 PCR 进行。结果:与同一患者的正常甲状腺组织相比,miR-146b、miR-181a、miR-187、miR-221 和 miR-222 在 PTC 中上调。与结节性甲状腺肿相比,miR-146b、miR-187、miR-221 和 miR-222 在 PTC 中也上调。复发性肿瘤与 miR-221 的上调在统计学上显着相关。BRAF基因的突变V600E与miR-146b的上调和miR-187的下调显着相关。结论:与正常甲状腺组织和结节性甲状腺肿相比,发现 PTC 中选定的 miRNA 过度表达。此外,miR-221 可作为预后标志物,因为其过表达与复发性肿瘤显着相关。与同一患者的正常甲状腺组织相比,miR-146b、miR-181a、miR-187、miR-221 和 miR-222 在 PTC 中上调。与结节性甲状腺肿相比,miR-146b、miR-187、miR-221 和 miR-222 在 PTC 中也上调。复发性肿瘤与 miR-221 的上调在统计学上显着相关。BRAF基因的突变V600E与miR-146b的上调和miR-187的下调显着相关。结论:与正常甲状腺组织和结节性甲状腺肿相比,发现 PTC 中选定的 miRNA 过度表达。此外,miR-221 可作为预后标志物,因为其过表达与复发性肿瘤显着相关。与同一患者的正常甲状腺组织相比,miR-146b、miR-181a、miR-187、miR-221 和 miR-222 在 PTC 中上调。与结节性甲状腺肿相比,miR-146b、miR-187、miR-221 和 miR-222 在 PTC 中也上调。复发性肿瘤与 miR-221 的上调在统计学上显着相关。BRAF基因的突变V600E与miR-146b的上调和miR-187的下调显着相关。结论:与正常甲状腺组织和结节性甲状腺肿相比,发现 PTC 中选定的 miRNA 过度表达。此外,miR-221 可作为预后标志物,因为其过表达与复发性肿瘤显着相关。与结节性甲状腺肿相比,miR-221 和 miR-222 在 PTC 中也上调。复发性肿瘤与 miR-221 的上调在统计学上显着相关。BRAF基因的突变V600E与miR-146b的上调和miR-187的下调显着相关。结论:与正常甲状腺组织和结节性甲状腺肿相比,发现 PTC 中选定的 miRNA 过度表达。此外,miR-221 可作为预后标志物,因为其过表达与复发性肿瘤显着相关。与结节性甲状腺肿相比,miR-221 和 miR-222 在 PTC 中也上调。复发性肿瘤与 miR-221 的上调在统计学上显着相关。BRAF基因的突变V600E与miR-146b的上调和miR-187的下调显着相关。结论:与正常甲状腺组织和结节性甲状腺肿相比,发现 PTC 中选定的 miRNA 过度表达。此外,miR-221 可作为预后标志物,因为其过表达与复发性肿瘤显着相关。结论:与正常甲状腺组织和结节性甲状腺肿相比,发现 PTC 中选定的 miRNA 过度表达。此外,miR-221 可作为预后标志物,因为其过表达与复发性肿瘤显着相关。结论:与正常甲状腺组织和结节性甲状腺肿相比,发现 PTC 中选定的 miRNA 过度表达。此外,miR-221 可作为预后标志物,因为其过表达与复发性肿瘤显着相关。
更新日期:2021-01-01
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