Sleep homeostasis is crucial for sleep regulation. The role of epigenetic regulation in sleep homeostasis is unestablished. Previous studies showed that octopamine is important for sleep homeostasis. However, the regulatory mechanism of octopamine reception in sleep is unknown. In this study, we identify an epigenetic regulatory cascade (Stuxnet‐Polycomb‐Octβ2R) that modulates the octopamine receptor in Drosophila. We demonstrate that stuxnet positively regulates Octβ2R through repression of Polycomb in the ellipsoid body of the adult fly brain and that Octβ2R is one of the major receptors mediating octopamine function in sleep homeostasis. In response to octopamine, Octβ2R transcription is inhibited as a result of stuxnet downregulation. This feedback through the Stuxnet‐Polycomb‐Octβ2R cascade is crucial for sleep homeostasis regulation. This study demonstrates a Stuxnet‐Polycomb‐Octβ2R‐mediated epigenetic regulatory mechanism for octopamine reception, thus providing an example of epigenetic regulation of sleep homeostasis.

中文翻译：

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表观遗传调节因子 Stuxnet 通过 Stuxnet-Polycomb-Octβ2R 级联调节章鱼胺对睡眠的影响

睡眠稳态对于睡眠调节至关重要。表观遗传调控在睡眠稳态中的作用尚未确定。先前的研究表明，章鱼胺对睡眠稳态很重要。然而，睡眠中章鱼胺接收的调节机制尚不清楚。在这项研究中，我们发现了一种表观遗传调控级联反应（Stuxnet-Polycomb-Octβ2R），它可以调节果蝇中的章鱼胺受体。我们证明，stuxnet通过抑制成虫大脑椭圆体中的 Polycomb正向调节Octβ2R ，并且 Octβ2R 是调节睡眠稳态中章鱼胺功能的主要受体之一。作为对章鱼胺的反应，Octβ2R转录由于以下原因而受到抑制Stuxnet下调。这种通过 Stuxnet-Polycomb-Octβ2R 级联反应的反馈对于睡眠稳态调节至关重要。这项研究证明了 Stuxnet-Polycomb-Octβ2R 介导的章鱼胺接收的表观遗传调控机制，从而提供了睡眠稳态的表观遗传调控的一个例子。