Omics technologies, such as genomics, epigenomics, transcriptomics, proteomics, metabolomics, lipidomics, multiomics, and integrated modalities, have greatly contributed to our understanding of various diseases by enabling researchers to probe the molecular wiring of cellular systems in a high-throughput and precise manner. With the development of tissue-engineered three-dimensional (3D) in vitro disease models, such as organoids and spheroids, there is potential of integrating omics technologies with 3D disease models to elucidate the complex links between genotype and phenotype. These 3D disease models have been used to model cancer, infectious disease, toxicity, neurological disorders, and others. In this review, we provide an overview of omics technologies, highlight current and emerging studies, discuss the associated experimental design considerations, barriers and challenges of omics technologies, and provide an outlook on the future applications of omics technologies with 3D models. Overall, this review aims to provide a valuable resource for tissue engineers seeking to leverage omics technologies for diving deeper into biological discovery.

中文翻译：

---

组学技术在三维体外疾病模型中的应用

组学技术，如基因组学、表观基因组学、转录组学、蛋白质组学、代谢组学、脂质组学、多组学和综合模式，通过使研究人员能够以高通量和精确的方式探测细胞系统的分子布线，极大地促进了我们对各种疾病的理解。方式。随着体外组织工程三维（3D）技术的发展疾病模型，如类器官和球体，有可能将组学技术与 3D 疾病模型相结合，以阐明基因型和表型之间的复杂联系。这些 3D 疾病模型已用于对癌症、传染病、毒性、神经系统疾病等进行建模。在这篇综述中，我们概述了组学技术，重点介绍了当前和新兴的研究，讨论了相关的实验设计考虑因素、组学技术的障碍和挑战，并展望了 3D 模型组学技术的未来应用。总体而言，本综述旨在为寻求利用组学技术深入研究生物发现的组织工程师提供宝贵的资源。