The aim of this study is to compare different methods due to the difficulties in identifying coryneform bacteria to species level and to determine antibiotic resistance profiles. Isolates identified as Turicella otitidis (n:45) by VITEK 2 Compact and Corynebacterium mucifaciens (n:1) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), isolated from blood and catheter cultures between 2015 and 2017 were included in the study. For identification of the isolates, conventional tests and 16S rDNA sequence analysis were performed. Antibiotic susceptibilities of the isolates were determined by Etest. The isolates identified as T. otitidis with VITEK 2 Compact could not be identified by MALDI-TOF MS and described as C. mucifaciens/Corynebacterium afermentans spp. by 16S rDNA sequence analysis. One isolate identified as C. mucifaciens by MALDI-TOF MS could not be identified with VITEK 2 Compact and described as C. mucifaciens by 16S rDNA sequence analysis and conventional methods. All isolates (n:45) described as C. mucifaciens/C. afermentans spp. by 16S rDNA sequence analysis were identified as C. afermentans subsp. afermentans with conventional methods. All 45 isolates identified as C. afermentans subsp. afermentans were resistant to penicillin, erythromycin, and clindamycin and were susceptible to vancomycin and daptomycin, whereas 31 (69%) were resistant to trimethoprim-sulfamethoxazole (TMP-SXT). The isolate identified as C. mucifaciens was susceptible to penicillin, vancomycin, daptomycin, and TMP-SXT; it was resistant to erythromycin and clindamycin. In this study, we reported 45 C. afermentans isolates misidentified as T. otitidis in routine laboratory processes. To our knowledge, this is the first study to include the highest number of C. afermentans blood isolates.

中文翻译：

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传统方法、自动化系统和 DNA 序列分析方法在鉴定从血液和导管培养样品中分离的棒状杆菌和黏液棒状杆菌细菌的比较

本研究的目的是比较不同的方法，因为在物种水平上识别棒状细菌和确定抗生素抗性谱存在困难。通过基质辅助激光解吸电离飞行时间质谱 (MALDI-TOF MS) 从血液和导管培养物中分离出的分离株被 VITEK 2 Compact 和粘液棒状杆菌( n :1)鉴定为耳炎风疹( Turicella otitidis) ( n :45) 2015 年和 2017 年被纳入研究。为了鉴定分离株，进行了常规测试和 16S rDNA 序列分析。通过Etest测定分离株的抗生素敏感性。被鉴定为耳炎滴虫的分离株使用 VITEK 2 Compact 无法通过 MALDI-TOF MS 进行鉴定，并被描述为粘液梭菌/棒状杆菌属。通过 16S rDNA 序列分析。一种被 MALDI-TOF MS鉴定为粘液梭菌的分离株无法使用 VITEK 2 Compact 进行鉴定，并通过 16S rDNA 序列分析和常规方法被描述为粘液梭菌。所有分离株 ( n :45) 被描述为C. mucifaciens / C. afermentans spp。通过 16S rDNA 序列分析被鉴定为C. afermentans subsp。用传统方法发酵。所有 45 个分离株均被鉴定为C. afermentans subsp。afermentans对青霉素、红霉素和克林霉素耐药，对万古霉素和达托霉素敏感，而 31 (69%) 对甲氧苄啶-磺胺甲恶唑 (TMP-SXT) 耐药。被鉴定为粘液梭菌的分离株对青霉素、万古霉素、达托霉素和 TMP-SXT 敏感；对红霉素和克林霉素耐药。在这项研究中，我们报告了 45 个C. afermentans分离株，在常规实验室过程中被误认为是T. otitidis。据我们所知，这是第一项包括最高数量的C. afermentans血液分离株的研究。