Clostridium perfringens is a key anaerobic pathogen causing food poisoning. Definitive detection by standard culture method is time-consuming and labor intensive. Current rapid commercial test kits are prohibitively expensive. It is thus necessary to develop rapid and cost-effective detection tool. Here, loop-mediated isothermal amplification (LAMP) in combination with a lateral-flow biosensor (LFB) was developed for visual inspection of C. perfringens-specific cpa gene. The specificity of the developed test was evaluated against 40 C. perfringens and 35 other bacterial strains, which showed no cross-reactivity, indicating 100% inclusivity and exclusivity. LAMP-LFB detection limit for artificially contaminated samples after enrichment for 16 h was 1–10 CFU/g sample, which was comparable to the commercial real-time PCR kit. The detection performance of LAMP-LFB was also compared to culture-based method using 95 food samples, which revealed the sensitivity (SE), specificity (SP) and Cohen's kappa coefficient (κ) of 88.0% (95% CI, 75.6%-95.4%), 95.5% (95% CI, 84.8%-99.4%) and 0.832 (95% CI, 0.721–0.943), respectively. Area under the receiver operating characteristic (ROC) curve was 0.918 (95% CI, 0.854–0.981), indicating LAMP-LFB as high relative accuracy test. In conclusion, LAMP-LFB assay is a low-cost qualitative method and easily available for routine detection of C. perfringens in food samples, which could serve as an alternative to commercial test kit.

产气荚膜梭状芽胞杆菌是导致食物中毒的关键厌氧性病原体。用标准培养方法进行确定的检测是费时且费力的。当前的快速商业测试套件非常昂贵。因此，有必要开发一种快速且具有成本效益的检测工具。在此，在与侧流生物传感器（LFB）组合环介导等温扩增（LAMP）被用于视觉检查开发Ç。产气荚膜特异性cpa基因。针对40 C评估开发的测试的特异性。产气荚膜菌其他35个细菌菌株没有交叉反应，表明包容性和排他性均为100％。富集16 h后，人工污染样品的LAMP-LFB检测极限为1–10 CFU / g样品，与商业实时PCR试剂盒相当。还比较了LAMP-LFB的检测性能与基于培养的方法（使用95个食品样品的检测方法）的敏感性，结果显示，其灵敏度（SE），特异性（SP）和科恩卡伯系数（κ）为88.0％（95％CI，75.6％- 95.4％），95.5％（95％CI，84.8％-99.4％）和0.832（95％CI，0.721-0.943）。接收器工作特性（ROC）曲线下的面积为0.918（95％CI，0.854-0.981），表明LAMP-LFB是高相对精度测试。总之，LAMP-LFB测定是一种低成本的定性方法，可轻松用于常规检测Ç。食品样品中的香水成分，可作为商业检测试剂盒的替代品。