In this paper, we explored a rapid and ultra-sensitive detection method of fucoxanthin in algae by surface-enhanced Raman scattering. Gold nanoparticles (Au NPs) were prepared by the reduction of chloroauric acid with citric acid. An effective Raman indicator molecule, Rhodamine 6G(R6G) dye molecule, was used to characterize Au NPs that were used in SERS analysis to detect fucoxanthin. It was found that the detection limit of fucoxanthin could reach 10⁻⁶ mol·L⁻¹. The characteristic peak of fucoxanthin at a wave number of 1520 cm⁻¹was selected as the working curve, and it presents good linearity of fucoxanthin concentration with R² = 0.932 in the range from 5×10⁻⁴∼1×10⁻⁷ M. We first proposed the rapid detection of fucoxanthin by surface-enhanced Raman scattering, which took only one minute to detect. In addition, the Raman strength of the pure algae or kelp and the addition of fucoxanthin was compared under the gold nanoparticle substrate, so as to verify the application value of SERS technology in the rapid determination of fucoxanthin.

中文翻译：

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金纳米颗粒快速，灵敏，无标记的SERS测定藻类中的岩藻黄质

在本文中，我们探索了通过表面增强拉曼散射法快速，超灵敏地检测藻类中岩藻黄质的方法。通过用柠檬酸还原氯金酸来制备金纳米颗粒（Au NPs）。有效的拉曼指示剂分子，若丹明6G（R6G）染料分子，用于表征Au NP，用于SERS分析以检测岩藻黄质。结果发现，藻褐素的检测限可达到10 ^-6莫尔^-1。在1520厘米的波数褐藻素的特征峰^-1被选定为工作曲线，并将其呈现有R褐藻素浓度的良好的线性² 5×10在范围= 0.932 ^-4〜1×10 ^-7M.我们首先提出了通过表面增强拉曼散射快速检测岩藻黄质的方法，该方法只需一分钟即可检测到。另外，在金纳米颗粒底物下比较了纯藻类或海带的拉曼强度和岩藻黄质的添加量，从而验证了SERS技术在岩藻黄质快速测定中的应用价值。