Common vetch (Vicia sativa) is an important forage and green manure crop that is widely cultivated around the world. However, the large number of subspecies and accessions of common vetch has made taxonomic research on this species difficult. Pollen morphology data can provide important evidence in the study of plant phylogeny. Therefore, in this research, light microscopy and scanning electron microscopy were used to observe seven morphological traits of pollens from 22 common vetch accessions, and residual maximum likelihood and pattern analysis was conducted. The results showed that the pollen grains of the 22 accessions were all monad pollen and the polar view revealed three-lobed circular and triangular shapes, while the equatorial view mainly revealed an oblate shape; only one accession showed an oblate spherical shape. All accessions were 3-zonocolporate and the colpus length extended close to the poles. The polar axis length was (19.39 ± 0.97)–(42.12 ± 0.76) μm and the equatorial axis length was (35.97 ± 1.27)–(45.25 ± 0.81) μm. We found that qualitative traits of pollen shape, aperture polar view and ornamentation were highly stable. Among them, polar axis length, equatorial axis length and colpus length and width had significant differences among the accessions. The ratios of the equatorial and polar axes had significant differences among the subspecies. This trait information could be used for the classification and identification of common vetch accessions and subspecies.

中文翻译：

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普通野豌豆（Vicia sativa）亚种花粉形态的扫描电子显微镜观察

紫云英（苜蓿) 是一种重要的牧草和绿肥作物，在世界各地广泛种植。然而，紫云英的大量亚种和种质使得对该种的分类学研究变得困难。花粉形态数据可为植物系统发育研究提供重要依据。因此，本研究采用光学显微镜和扫描电子显微镜观察了22个紫云英种质花粉的7个形态特征，并进行了残差最大似然和模式分析。结果表明，22份种质的花粉粒均为单子花粉，极面呈三裂圆形和三角形，赤道面主要呈扁圆形；只有一个种质呈扁球形。所有种质都是 3-zonocolporate 并且 colpus 长度延伸到靠近两极。极轴长度为（19.39±0.97）-（42.12±0.76）μm，赤道轴长度为（35.97±1.27）-（45.25±0.81）μm。我们发现花粉形状、孔径极视图和纹饰的质量性状高度稳定。其中，极轴长度、赤道轴长度和colpus长度和宽度在种质之间存在显着差异。赤道轴和极轴的比值在亚种间存在显着差异。该性状信息可用于普通紫云英种质和亚种的分类和鉴定。我们发现花粉形状、孔径极视图和纹饰的质量性状高度稳定。其中，极轴长度、赤道轴长度和colpus长度和宽度在种质之间存在显着差异。赤道轴和极轴的比值在亚种间存在显着差异。该性状信息可用于普通紫云英种质和亚种的分类和鉴定。我们发现花粉形状、孔径极视图和纹饰的质量性状高度稳定。其中，极轴长度、赤道轴长度和colpus长度和宽度在种质之间存在显着差异。赤道轴和极轴的比值在亚种间存在显着差异。该性状信息可用于普通紫云英种质和亚种的分类和鉴定。