The direct and indirect competition time-resolved fluorescence immunoassays (dc-TRFIA, ic-TRFIA) were established by combining the autofluorescence properties of lanthanide europium (Eu) with the monoclonal antibody of oxyfluorfen. The purified Eu antibody was optimized and the conditions such as the working concentration of the Eu antibody, monoclonal antibody, and working buffer were optimized. In the optimal condition, the IC₅₀ of dc-TRFIA was 10.27 ng/mL, the lowest detection limit IC₁₀ was 0.071 ng/mL, the detection range (IC₁₀-IC₉₀) was 0.071–1074.3 ng/mL, and the detection range (IC₁₀-IC₉₀) and IC₅₀ of ic-TRFIA were 0.024–504.6 and 2.76 ng/mL, respectively. The comparison showed that the sensitivity and detection limit of ic-TRFIA were superior to dc-TRFIA. The cross reaction (CR) tests showed that the CR with other oxyfluorfen structure analogs was <0.02%, except that there was a certain CR with the benzofluorfen (CR = 11.58) and the bifenox (CR = 8.23%). The average recoveries of ic-TRFIA were 74.6–108.3%, and the RSDs were between 2.1 and 10.9%, in the addition recovery test with five substrates. The results of the correlation test with the real samples of GC-ECD showed that they were highly correlated (y = 0.975x – 0.4446, R² = 0.9901), which proved that the TRFIA method established in this study had high reliability and accuracy and could be used in environment and agricultural products for rapid detection of oxyfluorfen residues.

将镧系铕（Eu）的自发荧光特性与乙氧氟草醚单克隆抗体相结合，建立了直接和间接竞争时间分辨荧光免疫分析（dc-TRFIA、ic-TRFIA）。对纯化的Eu抗体进行了优化，并对Eu抗体的工作浓度、单克隆抗体、工作缓冲液等条件进行了优化。在最佳条件下，dc-TRFIA的IC ₅₀为10.27 ng/mL，最低检测限IC ₁₀为0.071 ng/mL，检测范围（IC ₁₀ -IC ₉₀ ）为0.071~1074.3 ng/mL， ic-TRFIA的检测范围(IC ₁₀ -IC ₉₀ )和IC ₅₀分别为0.024-504.6和2.76 ng/mL。比较表明，ic-TRFIA的灵敏度和检出限均优于dc-TRFIA。交叉反应（CR）测试表明，与其他乙氧氟草醚结构类似物的交叉反应<0 id=171>y = 0.975x – 0.4446，右² =0.9901），证明本研究建立的TRFIA方法具有较高的可靠性和准确性，可用于环境和农产品中乙氧氟草醚残留的快速检测。