This study aimed to investigate the antioxidant ability and the chemical composition of apolar and polar extractives from Douglas-fir bark, cork and phloem, establishing a possible correlation with the structural variation along the tree stem and geographic location. Douglas-fir bark extractives’ composition were analyzed at three stem heights in trees from two locations. Cork and phloem extracts’ composition were analyzed in samples collected at stem base. Extractives content in Douglas-fir bark varied between 14 and 31% and polar extractives (11–29%) were dominant over non-polar. Lipophilic extracts were mainly composed of terpenoids, representing 27–77% of all compounds, highlighted by callitrisic acid (11–34%). Sterols were also abundant (6–45%), with β-sitosterol representing 7–33% of all compounds. Alkanoic acids were present in smaller amounts. Ethanol–water extracts showed high phenolic (562–762 mg GAE/g extract), flavonoid and condensed tannins contents (399–683 mg CE/g extract and 120–262 mg CE/g of extract), high scavenging (IC 50 2.8 µg extract/mL) and reducing (12 mM Fe 2+ /g extract) abilities. Cork had high phenolic (819 mg GAE/g extract) and flavonoid contents (524 mg CE/g extract) and high antioxidant capacity (1080 mg TEAC/g extract). Detailed knowledge of Douglas-fir extracts demonstrates their potential as a source of fine chemicals towards different applications.

中文翻译：

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花旗松树皮提取物的组成和抗氧化特性

本研究旨在研究花旗松树皮、软木和韧皮部的非极性和极性提取物的抗氧化能力和化学成分，建立与树干结构变异和地理位置的可能相关性。花旗松树皮提取物的成分在来自两个位置的树木的三个茎高处进行了分析。在茎基部收集的样品中分析了软木和韧皮部提取物的组成。花旗松树皮中的提取物含量在 14% 到 31% 之间变化，极性提取物（11-29%）比非极性提取物占主导地位。亲脂性提取物主要由萜类化合物组成，占所有化合物的 27-77%，以 callitrisic 酸 (11-34%) 突出。甾醇也很丰富（6-45%），β-谷甾醇占所有化合物的 7-33%。链烷酸以较少量存在。乙醇-水提取物显示出高酚类（562-762 mg GAE/g 提取物）、类黄酮和缩合单宁含量（399-683 mg CE/g 提取物和 120-262 mg CE/g 提取物）、高清除率（IC 50 2.8 µg 提取物/mL）和还原（12 mM Fe 2+ /g 提取物）能力。软木具有高酚类（819 mg GAE/g 提取物）和类黄酮含量（524 mg CE/g 提取物）和高抗氧化能力（1080 mg TEAC/g 提取物）。花旗松提取物的详细知识证明了它们作为精细化学品来源用于不同应用的潜力。软木具有高酚类（819 mg GAE/g 提取物）和类黄酮含量（524 mg CE/g 提取物）和高抗氧化能力（1080 mg TEAC/g 提取物）。花旗松提取物的详细知识证明了它们作为精细化学品来源用于不同应用的潜力。软木具有高酚类（819 mg GAE/g 提取物）和类黄酮含量（524 mg CE/g 提取物）和高抗氧化能力（1080 mg TEAC/g 提取物）。花旗松提取物的详细知识证明了它们作为精细化学品来源用于不同应用的潜力。