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The effects of phosphate on physiological responses and carbohydrate production in Ulva fasciata (Chlorophyta) from upwelling and non-upwelling sites
Botanica Marina ( IF 2.2 ) Pub Date : 2021-02-01 , DOI: 10.1515/bot-2020-0051
Tiphane Andrade Figueira 1 , Nuno Tavares Martins 2 , Lígia Ayres-Ostrock 2 , Estela M. Plastino 2 , Alex Enrich-Prast 3 , Vinícius Peruzzi de Oliveira 1
Affiliation  

Phosphorus is a key macronutrient in macroalgal physiology, including carbohydrate anabolism. To determine the effects of phosphorus on different physiological parameters, we cultivated Ulva fasciata specimens from distinct localities (upwelling and non-upwelling sites) in the presence of different phosphate concentrations (0, 2, and 4 µM PO43−${\text{PO}}_{4}^{3-}$). After 15 days, growth rates were similar (approx. 12% day −1 ) and carbohydrate contents had increased in individuals from both sites. In individuals from the upwelling site, carbohydrate contents were high in all treatments (71% dry mass), whereas non-upwelling site individuals cultivated under the highest phosphate concentration showed the lowest carbohydrate content (46% DM). Nevertheless, we observed higher rates of phosphorus uptake in individuals from the non-upwelling site cultivated under the highest phosphate concentration, indicating a stress response to elevated nutrient concentrations. Individuals from both sites cultivated with phosphate maintained healthy photosystems over the experimental period ( F v / F m = 0.788), whereas those cultivated in the absence of phosphate showed evidence of photosystem impairment, as indicated by a progressive decline in maximum quantum yield. Altogether, our results indicate that site origin and phosphate concentration influence the carbohydrate content in U. fasciata and that individuals from sites with broad environmental variation, such as upwelling events, can show higher productivity.

中文翻译:

磷酸盐对上隆和非隆隆部位的阔叶锦囊藻(Chlorophyta)生理响应和碳水化合物产生的影响

磷是包括藻类合成代谢在内的大型藻类生理中的重要大型营养素。为了确定磷对不同生理参数的影响,我们在不同磷酸盐浓度(0、2和4 µM PO43 − $ {\ text {PO }} _ {4} ^ {3-} $)。15天后,两个站点的个体的生长速率相似(约-1天-12%),碳水化合物含量增加。在来自上升流部位的个体中,所有处理中的碳水化合物含量均较高(干质量的71%),而在最高磷酸盐浓度下培养的非上升流部位的个体显示最低的碳水化合物含量(46%DM)。尽管如此,我们观察到在磷酸盐浓度最高的情况下,来自非上升丘陵地的个体吸收磷的比率更高,这表明对营养物浓度升高的胁迫反应。在实验期间,两个地点都用磷酸盐栽培的个体保持健康的光系统(F v / F m = 0.788),而在没有磷酸盐的情况下栽培的个体显示出光系统受损的迹象,如最大量子产率的逐渐下降所表明的。总之,我们的结果表明位点起源和磷酸盐浓度会影响筋膜藻中的碳水化合物含量,并且来自环境变化较大的位点(例如上升流事件)的个体可以显示出更高的生产力。表明对营养物浓度升高的压力反应。在实验期间,两个地点都用磷酸盐栽培的个体保持健康的光系统(F v / F m = 0.788),而在没有磷酸盐的情况下栽培的个体显示出光系统受损的迹象,如最大量子产率的逐渐下降所表明的。总之,我们的结果表明位点起源和磷酸盐浓度会影响筋膜藻中的碳水化合物含量,并且来自环境变化较大的位点(例如上升流事件)的个体可以显示出更高的生产力。表明对营养物浓度升高的压力反应。在实验期间,两个地点都用磷酸盐栽培的个体保持健康的光系统(F v / F m = 0.788),而在没有磷酸盐的情况下栽培的个体显示出光系统受损的迹象,如最大量子产率的逐渐下降所表明的。总之,我们的结果表明位点起源和磷酸盐浓度会影响筋膜藻中的碳水化合物含量,并且来自环境变化较大的位点(例如上升流事件)的个体可以显示出更高的生产力。而在没有磷酸盐的情况下耕种的植物则显示出光系统受损的迹象,如最大量子产率的逐渐下降所表明的。总之,我们的结果表明位点起源和磷酸盐浓度会影响筋膜藻中的碳水化合物含量,并且来自环境变化较大的位点(例如上升流事件)的个体可以显示出更高的生产力。而在没有磷酸盐的情况下耕种的植物则显示出光系统受损的迹象,如最大量子产率的逐渐下降所表明的。总之,我们的结果表明位点起源和磷酸盐浓度会影响筋膜藻中的碳水化合物含量,并且来自环境变化较大的位点(例如上升流事件)的个体可以显示出更高的生产力。
更新日期:2021-03-16
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