ABSTRACT

Capsicum annuum is also known as chili which is one of the most important vegetable crops grown in the world. Breeding new varieties with heterosis could improve the quality of pepper, increase yield, growth potential, disease resistance, adaptability, and seed viability. To investigate the heterosis among three cross combinations of different parents, the mRNA-miRNA integrated analysis was performed. A total number of 22,659,009 to 36,423,818 clean data were generated from mRNA-seq with 81 libraries, and the unique mapped reads were from 35,495,567 (86.81%) to 46,466,622 (88.95%). The plant-hormone signal transduction pathway (40 genes) was detected with a higher DEG number. The SAUR32L, GID1, PYR1, EIN2. ERF1, PR1, JAR1-like, IAA from this pathway play a key role in plant development. From the miRNA-seq, the number of clean reads was ranging from 12,132,221 to 25,632,680. A total of 220 miRNAs were predicted in this study, and all of them were identified as novel miRNA. The top three candidate KEGG pathways of miRNA were ribosome signaling pathway (13 miRNAs), spliceosome pathway (13 miRNAs), and plant hormone signal transduction pathways (10 miRNAs). With the mRNA and miRNA integrated analysis, we found some key genes were regulated by some miRNAs. Among them, the scarecrow-like 6 protein can be up or down regulated by mir8, mir120, mir184, mir_214, mir125, and mir130. The function of Della protein was regulated by mir24, mir74, mir94, mir139, and mir190. This study contributes to understanding how heterosis regulates the traits, such as crop production, fruit weight, and fruit length.

摘要

辣椒（辣椒）也被称为辣椒，它是世界上最重要的蔬菜作物之一。育种具有杂种优势的新品种可以提高辣椒的质量，提高产量，增长潜力，抗病性，适应性和种子活力。为了研究不同亲本的三个交叉组合之间的杂种优势，进行了mRNA-miRNA整合分析。从具有81个文库的mRNA-seq生成了22,659,009到36,423,818个干净数据，唯一的映射读段为35,495,567（86.81％）至46,466,622（88.95％）。检测到的植物激素信号转导途径（40个基因）的DEG数更高。该SAUR32L，GID1，PYR1，EIN2。ERF1，PR1，类似JAR1的IAA从这个途径中起着关键作用，在植物发育中。根据miRNA-seq，干净读取的数目为12,132,221至25,632,680。在这项研究中，总共预测了220个miRNA，并将它们全部鉴定为新型miRNA。miRNA的前三个候选KEGG途径是核糖体信号传导途径（13个miRNA），剪接体途径（13个miRNA）和植物激素信号转导途径（10个miRNA）。通过对mRNA和miRNA的整合分析，我们发现一些关键基因受到某些miRNA的调控。其中，稻草人样6蛋白可以由mir8，mir120，mir184，mir_214，mir125和mir130上调或下调。Della蛋白的功能由mir24，mir74，mir94，mir139和mir190调节。这项研究有助于了解杂种优势如何调节性状，例如农作物产量，果实重量，