Salt stress will seriously influence alt stress in banana (Musa L.) induces MaPIP1;1, which enhances the salt resistance of transgenic Arabidopsis. The promoter pMaPIP1;1, located 13,62 bp upstream of the transcriptional start site, was isolated from the banana genome, and four pMaPIP1;1::GUS fusion constructs namely M‐P1, M‐P2, M‐P3, and M‐P4 were used to transform into Arabidopsis. In this work, in order to functionally verify the promoter responds to high salt stress, we used NaCl treatment for simulating salt terms. NaCl treatment is responded to by four transformants. M‐P2 (−1,274 bp to −1) showed the highest transcriptional activity among all transgenic Arabidopsis transformants. This area of the promoter endows high glucuronidase (GUS) enzyme activity according to NaCl treatment. These results will help us understand the transcriptional regulatory of MaPIP1;1, and promoter fragment M‐P2 will be an ideal candidate for overexpression of salt response genes to increase plant resistance.

中文翻译：

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鉴定可赋予转基因拟南芥盐分胁迫诱导能力的Mus acuminata L. aporporin基因（MaPIP1; 1）的1274-bp启动子

盐胁迫将严重影响香蕉（Musa L.）的高盐胁迫诱导MaPIP1; 1，可增强转基因拟南芥的耐盐性。从香蕉基因组中分离出了位于转录起始位点上游13,62 bp的启动子pMaPIP1; 1和四个pMaPIP1; 1 :: GUS融合构建体，即M‐P1，M‐P2，M‐P3和M -P4用于转化为拟南芥。在这项工作中，为了从功能上验证启动子对高盐胁迫的响应，我们使用了NaCl处理来模拟盐项。NaCl处理由四个转化子响应。在所有转基因拟南芥转化子中，M-P2（-1,274 bp至-1）显示出最高的转录活性。根据NaCl处理，该启动子区域赋予了高葡糖醛酸糖苷酶（GUS）酶活性。这些结果将有助于我们了解MaPIP1的转录调控； 1，启动子片段M-P2将是盐反应基因过表达以增加植物抗性的理想候选者。