It is known that miRNAs are effective in immune response in the diagnosis and treatment of many infectious diseases. However, the miRNAs profile is unknown in Alveolar and Cystic Echinococcosis which can be fatal if left untreated. The miRNAs profile that activates the T and B cells forming the immune system in Alveolar and Cystic Echinococcosis patients was investigated in this study. A total of 50 liver tissue samples were obtained from Alveolar and Cystic Echinococcosis patients in Kilis State Hospital Pathology Laboratory in southeast of Turkey. The circulating cell-free miRNAs were evaluated by a quantitative real-time polymerase chain reaction, statistically calculated within ΔΔCt values and fold changes were evaluated by Welch T test, in which P < .05 was considered to be significant. Twenty-five microRNAs, including let-7a-5p, let-7c, let-7e-5p, miR-15b-5p, miR16, miR-17-5p, miR-23a-5p, miR-24-3p, miR-25-3p, miR-26a-3p, miR-26b-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-30e-5p, miR-98-5p, miR-101-3p, miR-106b-5p, miR-125b-5p, miR-142-5p, miR-222-3p and miR-223-3p, were found as down-regulated in Alveolar and Cystic Echinococcosis patients than control groups. Twelve miRNAs, including miR-15a-5p, miR-21-5p, miR-27a-3p, miR-29a-3p, miR-146a-5p, miR-181a-5p, miR-181b-5p, miR-181d, miR-181c-5p, miR-195-5p, miR-214-3p and miR-365-3p, were found as up-regulated in Alveolar and Cystic Echinococcosis patients than healthy person. It has been shown that T- and B-cell activities are related in the progressive of both Alveolar and Cystic Echinococcosis in this study. The miRNA panel activated by T and B cells may be important for exploring the mechanisms underlying early development in Alveolar and Cystic Echinococcosis providing novel information that may be used to discover new therapeutics for these diseases.

中文翻译：

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肺泡和囊性棘球蚴病患者免疫反应中的 microRNA 谱

众所周知，miRNA在许多传染病的诊断和治疗中的免疫反应中是有效的。然而，在肺泡型和囊性棘球蚴病中，miRNAs 谱是未知的，如果不及时治疗可能会致命。本研究调查了激活 T 细胞和 B 细胞在肺泡和囊性棘球蚴病患者中形成免疫系统的 miRNA 谱。从土耳其东南部基利斯州立医院病理学实验室的肺泡和囊性棘球蚴病患者中获得了总共 50 份肝组织样本。通过定量实时聚合酶链反应评估循环无细胞 miRNA，在 ΔΔCt 值内进行统计计算，并通过 Welch T检验评估倍数变化，其中P < .05 被认为是显着的。25 种 microRNA，包括 let-7a-5p、let-7c、let-7e-5p、miR-15b-5p、miR16、miR-17-5p、miR-23a-5p、miR-24-3p、miR- 25-3p、miR-26a-3p、miR-26b-3p、miR-29b-3p、miR-29c-3p、miR-30a-5p、miR-30b-5p、miR-30c-5p、miR-30d- 5p、miR-30e-5p、miR-98-5p、miR-101-3p、miR-106b-5p、miR-125b-5p、miR-142-5p、miR-222-3p和miR-223-3p，发现在肺泡型和囊性棘球蚴病患者中比对照组下调。十二种miRNA，包括miR-15a-5p、miR-21-5p、miR-27a-3p、miR-29a-3p、miR-146a-5p、miR-181a-5p、miR-181b-5p、miR-181d、 miR-181c-5p、miR-195-5p、miR-214-3p 和 miR-365-3p 在肺泡型和囊型包虫病患者中的表达高于健康人。本研究表明，T 细胞和 B 细胞活动与肺泡型和囊性棘球蚴病的进展有关。由 T 细胞和 B 细胞激活的 miRNA 面板可能对于探索肺泡和囊性棘球蚴病早期发展的机制很重要，提供了可用于发现这些疾病的新疗法的新信息。