MagR (IscA1) is a member of the iron–sulphur cluster assembly proteins, which plays vital roles in many physiological processes, such as energy metabolism, electron transfer, iron homeostasis, heme biosynthesis and physiologically magnetic response. Its deletion leads to the loss of mitochondrial DNA, inactivation of iron–sulphur proteins and abnormal embryonic development in organisms. However, the physiological roles of MagR in insects are unclear. This study characterized the effects and molecular regulatory mechanism of MagR gene silencing on the reproduction of brachypterous female adults of Nilaparvata lugens. After silencing the MagR gene using RNAi approach, the duration of reproductive period was shortened and the fecundity and hatchability reduced significantly. A total of 479 differentially expressed genes (DEGs) were identified for female adults after 2 days of dsRNA injection through RNA‐sequencing technology, including 352 significantly upregulated DEGs and 127 significantly downregulated DEGs, among which 44 DEGs were considered the key genes involved in the effects of NlMagR silencing on the reproduction, revealing the regulatory mechanism of MagR at RNA transcription level and providing a new strategy for the control of N. lugens.

中文翻译：

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转录组学分析显示，在沉默 MagR (IscA1) 基因后，水稻褐飞虱 Nilaparvata lugens 的繁殖受到抑制

MagR (IscA1) 是铁硫簇组装蛋白的成员，在许多生理过程中起着至关重要的作用，如能量代谢、电子转移、铁稳态、血红素生物合成和生理磁反应。它的缺失导致线粒体 DNA 的丢失、铁硫蛋白的失活和生物体的异常胚胎发育。然而，MagR 在昆虫中的生理作用尚不清楚。本研究表征了 MagR 基因沉默对Nilaparvata lugens短翅雌性成虫繁殖的影响和分子调控机制. 采用RNAi方法沉默MagR基因后，繁殖期持续时间缩短，繁殖力和孵化率显着降低。通过RNA测序技术，在注射dsRNA 2天后，共鉴定出雌性成人479个差异表达基因（DEGs），其中352个显着上调，127个显着下调，其中44个被认为是参与发育的关键基因。的影响NlMagR沉默的再现，揭示MAGR在RNA转录水平的调控机制和控制提供了一个新的战略褐飞虱。