The present study was conducted to investigate the possible lipid metabolism regulatory function of fenofibrate in obscure puffer fed with different dietary lipid levels. Obscure puffer were fed a low‐fat diet (LFD, 38.0 g/kg fat), medium‐fat diet (MFD, 132.0 g/kg) or high‐fat diet (HFD, 223.0 g/kg) for 10 weeks, and then fed diets supplemented with or without fenofibrate for 2 weeks. The results demonstrated that the final mean weight, hepatosomatic index (HSI), viscera index (VSI) and serum triglycerides (TG), cholesterol (CHO) levels increased, and the expression of pparα and some lipolysis‐related genes were up‐regulated as dietary lipid level increased. Dietary fenofibrate supplementation had no significant lipid‐lowering effect on fish fed with low or medium‐fat diets but significantly induced the expression of acox1. In contrast, fenofibrate treatment exhibited lipid‐lowering effect on obscure puffer fed with high‐fat diets, including significantly decreased the whole body crude lipid content, serum TG, CHO contents and hepatocyte lipid droplets size. Furthermore, dietary fenofibrate treatment significantly increased the expression of genes related to lipolysis (pparα, acox1, cpt1 and lpl) and lipogenesis (fas and pparγ) when fish were fed with high‐fat diets. Taken together, our present study suggested that the lipid‐lowering effects of fenofibrate were affected by the nutritional background of obscure puffer.

中文翻译：

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非诺贝特对暗淡河豚（Takifugu obscurus）脂质代谢的调节取决于饮食中的脂质含量

本研究旨在研究非诺贝特在不同饮食脂质水平下喂养的隐匿性河豚中可能的脂质代谢调节功能。给予不起眼的河豚低脂饮食（LFD，38.0 g / kg脂肪），中脂饮食（MFD，132.0 g / kg）或高脂饮食（HFD，223.0 g / kg）喂养10周，然后补充或不联合非诺贝特的饮食为期2周。结果表明，最终平均体重，肝体指数（HSI），内脏指数（VSI）和血清甘油三酸酯（TG），胆固醇（CHO）水平增加，并且pparα的表达随着饮食中脂质水平的升高，一些与脂解相关的基因也被上调。日粮非诺贝特补充剂对低脂或中脂饲料喂养的鱼没有明显的降脂作用，但能显着诱导acox1的表达。相比之下，非诺贝特治疗对高脂饮食喂养的晦涩河豚显示出降脂作用，包括显着降低了人体的粗脂肪含量，血清TG，CHO含量和肝细胞脂质滴大小。此外，饮食中的非诺贝特治疗可显着增加与脂肪分解相关的基因（pparα，acox1，cpt1和lpl）和脂肪形成（fas和pparγ）的表达。）以高脂饮食喂养鱼时。综上所述，我们的研究表明非诺贝特的降脂作用受到晦涩的河豚的营养背景的影响。