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Ultrasensitive Point‐of‐Care Test for Tumor Marker in Human Saliva Based on Luminescence‐Amplification Strategy of Lanthanide Nanoprobes
Advanced Science ( IF 14.3 ) Pub Date : 2021-01-06 , DOI: 10.1002/advs.202002657 Shanyong Zhou 1, 2 , Datao Tu 1, 2 , Yan Liu 1, 2 , Wenwu You 1 , Yunqin Zhang 1 , Wei Zheng 1, 2 , Xueyuan Chen 1, 2
Advanced Science ( IF 14.3 ) Pub Date : 2021-01-06 , DOI: 10.1002/advs.202002657 Shanyong Zhou 1, 2 , Datao Tu 1, 2 , Yan Liu 1, 2 , Wenwu You 1 , Yunqin Zhang 1 , Wei Zheng 1, 2 , Xueyuan Chen 1, 2
Affiliation
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The point‐of‐care detection of tumor markers in saliva with high sensitivity and specificity remains a daunting challenge in biomedical research and clinical applications. Herein, a facile and ultrasensitive detection of tumor marker in saliva based on luminescence‐amplification strategy of lanthanide nanoprobes is proposed. Eu2O3 nanocrystals are employed as bioprobes, which can be easily dissolved in acidic enhancer solution and transform into a large number of highly luminescent Eu3+ micelles. Meanwhile, disposable syringe filter equipped with nitrocellulose membrane is used as bioassay platform, which facilitates the accomplishment of detection process within 10 min. The rational integration of dissolution enhanced luminescent bioassay strategy and miniaturized detection device enables the unique lab‐in‐syringe assay of tumor marker like carcinoembryonic antigen (CEA, an important tumor marker in clinic diagnosis and prognosis of cancer) with a detection limit down to 1.47 pg mL−1 (7.35 × 10−15 m). Upon illumination with a portable UV flashlight, the photoluminescence intensity change above 0.1 ng mL−1 (0.5 × 10−12 m) of CEA can be visually detected by naked eyes, which allows one to qualitatively evaluate the CEA level. Moreover, we confirm the reliability of using the amplified luminescence of Eu2O3 nanoprobes for direct quantitation of CEA in patient saliva samples, thus validates the practicality of the proposed strategy for both clinical diagnosis and home self‐monitoring of tumor marker in human saliva.
中文翻译：
基于稀土纳米探针发光放大策略的人唾液中肿瘤标志物的超灵敏现场检测
高灵敏度和特异性地即时检测唾液中的肿瘤标志物仍然是生物医学研究和临床应用中的一项艰巨挑战。在此，提出了一种基于稀土纳米探针发光放大策略的唾液中肿瘤标志物的简便且超灵敏的检测。Eu 2 O 3纳米晶用作生物探针，它很容易溶解在酸性增强剂溶液中并转化为大量高发光的Eu 3+胶束。同时，采用配有硝酸纤维素膜的一次性注射器过滤器作为生物测定平台，有利于在10分钟内完成检测过程。溶出增强发光生物测定策略与小型化检测装置的合理结合，实现了癌胚抗原（CEA，癌症临床诊断和预后中重要的肿瘤标志物）等肿瘤标志物的独特注射器实验室测定，检测限低至1.47 pg mL −1 (7.35 × 10 −15 m )。在便携式紫外手电筒照射下，肉眼即可检测到CEA超过0.1 ng mL -1 (0.5 × 10 -12 m )的光致发光强度变化，从而可以定性评估CEA水平。此外，我们证实了使用Eu 2 O 3纳米探针的放大发光直接定量患者唾液样本中CEA的可靠性，从而验证了所提出的策略对于临床诊断和家庭自我监测人类唾液中肿瘤标志物的实用性。
更新日期：2021-03-03
中文翻译：
基于稀土纳米探针发光放大策略的人唾液中肿瘤标志物的超灵敏现场检测
高灵敏度和特异性地即时检测唾液中的肿瘤标志物仍然是生物医学研究和临床应用中的一项艰巨挑战。在此，提出了一种基于稀土纳米探针发光放大策略的唾液中肿瘤标志物的简便且超灵敏的检测。Eu 2 O 3纳米晶用作生物探针，它很容易溶解在酸性增强剂溶液中并转化为大量高发光的Eu 3+胶束。同时，采用配有硝酸纤维素膜的一次性注射器过滤器作为生物测定平台，有利于在10分钟内完成检测过程。溶出增强发光生物测定策略与小型化检测装置的合理结合，实现了癌胚抗原（CEA，癌症临床诊断和预后中重要的肿瘤标志物）等肿瘤标志物的独特注射器实验室测定，检测限低至1.47 pg mL −1 (7.35 × 10 −15 m )。在便携式紫外手电筒照射下，肉眼即可检测到CEA超过0.1 ng mL -1 (0.5 × 10 -12 m )的光致发光强度变化，从而可以定性评估CEA水平。此外，我们证实了使用Eu 2 O 3纳米探针的放大发光直接定量患者唾液样本中CEA的可靠性，从而验证了所提出的策略对于临床诊断和家庭自我监测人类唾液中肿瘤标志物的实用性。
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