Coronaviruses M proteins are well-represented in the major protein component of the viral envelope. During the viral assembly, they play an important role by association with all other viral structural proteins. Despite their crucial functions, very little information regarding the structures and functions of M proteins is available. Here we utilize bioinformatic tools from available sequences and 3D structures of SARS-CoV, SARS-CoV2, and MERS-CoV M proteins in order to predict potential B-cell epitopes and assessing antibody binding affinity. Such study aims to aid finding more effective vaccines and recognize neutralizing antibodies. we found some rather exciting differences between SARS-COV-2, SARS-Cov and MERS-CoV M proteins. Two SARS-CoV-2 peptides with significant antigen presentation scores for human cell surface proteins have been identified. The results reveal that N-terminal domains of M proteins of SARS-CoV and SARS-CoV2 are translocated (outside) whereas it is inside (cytoplasmic side) in MERS-CoV.

冠状病毒M 蛋白在病毒包膜的主要蛋白质成分中有很好的表现。在病毒组装过程中，它们通过与所有其他病毒结构蛋白结合发挥重要作用。尽管它们具有重要的功能，但关于 M 蛋白的结构和功能的信息却很少。在这里，我们利用来自 SARS-CoV、SARS-CoV2 和 MERS-CoV M 蛋白的可用序列和 3D 结构的生物信息学工具来预测潜在的 B 细胞表位并评估抗体结合亲和力。此类研究旨在帮助寻找更有效的疫苗并识别中和抗体。我们发现 SARS-COV-2、SARS-Cov 和 MERS-CoV M 蛋白之间存在一些相当令人兴奋的差异。已经鉴定出两种对人类细胞表面蛋白具有显着抗原呈递评分的 SARS-CoV-2 肽。