Localized hyperthermia is a very promising cancer therapy approach especially when stimulated by the exceptional properties of iron oxide magnetic nanoparticles (MNPs). This approach is a highly site-specific method for localized heating of bodily tissue without any harmful side effects that could revolutionize the practice of cancer therapy. The main objective of this study was to evaluate the cancer cell-destroying capability of MNPs in combination with ultrasound treatment as an innovative sonomagnetic cancer therapy. Magnetic nanofluids (MNFs) were synthesized by co-precipitation/sonochemical techniques in an aqueous medium without any surfactant and/or capping agent. The physicochemical characteristics of the prepared MNFs were investigated with scanning electron microscopy, transmission electron microscopy, X-ray diffractometry, Fourier transform infrared and vibrating sample magnetometry. The MNFs was used as a mediator and sonosensitizer to destroy tumor tissue when irradiated by ultrasound waves. The antitumor efficiency of MNFs in combination with pulsed ultrasound (1.5 W/cm², 1 MHz) was evaluated in vitro and in vivo. In vitro efficacy was estimated by determining the cell viability of Ehrlich ascites carcinoma cells. For in vivo experiments, female mice were inoculated subcutaneously with Ehrlich carcinoma cells to establish solid Ehrlich carcinoma. The cytotoxic concentration of MNFs (400 µg/mL) was injected intratumorally and exposed to pulsed ultrasound (1.5 W/cm², 1 MHz). The cytotoxic effect was determined in terms of tumor growth rate, apoptosis and necrosis. Our results revealed that MNFs in the presence of pulsed ultrasound cause a significant increase in the cytotoxicity effect on tumor cells. This study illustrates the high efficiency of cancer therapy as assisted by both ultrasound and magnetic nanofluid.

局部热疗是一种非常有前途的癌症治疗方法，尤其是在受到氧化铁磁性纳米粒子 (MNP) 的特殊特性刺激时。这种方法是一种局部加热身体组织的高度特定部位的方法，没有任何有害的副作用，可以彻底改变癌症治疗的实践。本研究的主要目的是评估 MNPs 结合超声治疗作为一种创新的声磁癌症治疗的癌细胞破坏能力。磁性纳米流体 (MNF) 是通过共沉淀/声化学技术在水性介质中合成的，没有任何表面活性剂和/或封端剂。用扫描电子显微镜、透射电子显微镜、X 射线衍射、傅里叶变换红外和振动样品磁强法。MNFs 被用作介质和声敏剂，在超声波照射时破坏肿瘤组织。MNFs 与脉冲超声 (1.5 W/cm² , 1 MHz)在体外和体内进行了评估。通过测定艾氏腹水癌细胞的细胞活力来估计体外功效。对于体内实验，雌性小鼠皮下接种埃利希癌细胞以建立实体埃利希癌。MNF 的细胞毒性浓度 (400 µg/mL) 被注射到肿瘤内并暴露于脉冲超声 (1.5 W/cm ², 1 兆赫）。根据肿瘤生长速率、细胞凋亡和坏死来确定细胞毒作用。我们的结果表明，在脉冲超声存在下，MNFs 导致对肿瘤细胞的细胞毒性作用显着增加。这项研究说明了在超声和磁性纳米流体的辅助下癌症治疗的高效性。