Significant improvements in the voltammetric determination of β-carotene (BCA) have been achieved, mainly by the replacement of toxic dichloromethane with acetone and using non-mercury electrode. The respective procedure is based on anodic oxidation of BCA at a gold electrode in the disc configuration, when using square-wave voltammetry in pure acetone (99.8%) with 0.1 mol L⁻¹ LiClO₄ as the supporting electrolyte. The method comprises extraction of the analyte from the sample with acetone, thus avoiding the usually used highly toxic solvents. Analytically, it can be characterized by a linear range from 6.0 × 10⁻⁶ to 5.9 × 10⁻⁴ mol L⁻¹ with regression equation I_p^a = 0.0184c −0.1631 and correlation coefficient, R² = 0.9998, limits of detection and quantification LOD = 1.6 × 10⁻⁶ mol L⁻¹ and LOQ = 5.4 × 10⁻⁶ mol L⁻¹, respectively; both being obtained at a potential step of 5 mV, with the pulse amplitude of 25 mV, and a frequency of 80 Hz. After optimization, the method was evaluated in series of analyses; namely, with two samples of vegetables and two pharmaceutical preparations (capsules), when the results could be compared to those of a reference spectrophotometric method. Due to a simple instrumentation, including sample preparation, the voltammetric method for the determination of BCA can be recommended as a quick screening assay in food and pharmaceutical analysis.

主要通过通过用丙酮代替有毒的二氯甲烷并使用非汞电极，在伏安法测定β-胡萝卜素（BCA）方面已取得了重大改进。当在具有0.1 mol L ^-1 LiClO ₄作为支持电解质的纯丙酮（99.8％）中使用方波伏安法时，相应的程序基于圆盘配置中金电极上BCA的阳极氧化。该方法包括用丙酮从样品中提取分析物，从而避免通常使用的剧毒溶剂。从分析上看，它的线性范围可以从6.0×10 ^-6到5.9×10 ^-4  mol L ^-1，具有回归方程I_p ^a  = 0.0184 c -0.1631和相关系数R ²  = 0.9998，检出限和定量限LOD = 1.6×10 ^-6  mol L ^-1和LOQ = 5.4×10 ^-6  mol L ^-1， 分别; 两者均以5 mV的电位阶跃，25 mV的脉冲幅度和80 Hz的频率获得。优化后，对该方法进行了一系列分析。即，可以将两个蔬菜样品和两个药物制剂（胶囊）样品的结果与参考分光光度法进行比较。由于包括样品制备在内的简单仪器，建议将伏安法测定BCA作为食品和药物分析中的快速筛选测定方法。