Acrylamide (AA) can have deleterious effects on freshwater fish. However, its adverse effects on euryhaline fish are still unknown. In this study, embryos of Oryzias melastigma were exposed to different concentrations of AA to investigate its effect on early developmental disorders. After 21 days of exposure, AA significantly inhibited the hatching rate and delayed the hatching time of embryos, and led to developmental delay, teratogenesis, and locomotion impairments in larvae. RNA-sequencing data of larvae indicated that AA upregulated the expression of hemoglobin and myoglobin involved in oxygen transport and angiopoietin 1, integrin, and matrix metallopeptidases related to angiogenesis and downregulated the expression of early growth response genes and synaptotagmin-2 related to neural plasticity and neurotransmitter release. Overall, our study showed that AA caused deleterious effects on the early development of euryhaline fish through hypoxic stress and neurotoxicity, providing a scientific basis for the environmental risk assessment of marine AA.

丙烯酰胺（AA）对淡水鱼有有害作用。但是，其对鱼腥鱼的不利影响仍然未知。在这项研究中，Oryzias melastigma的胚胎将他们暴露于不同浓度的AA中，以研究其对早期发育障碍的影响。暴露21天后，AA显着抑制了幼虫的孵化率并延迟了其孵化时间，并导致幼虫的发育延迟，致畸作用和运动障碍。幼虫的RNA测序数据表明，AA上调了与氧生成有关的血红蛋白和肌红蛋白的表达以及与血管生成有关的血管生成素1，整合素和基质金属肽酶的表达，并下调了与神经可塑性和相关性的早期生长反应基因和synaptotagmin-2的表达。神经递质释放。总体而言，我们的研究表明，AA会因缺氧应激和神经毒性而对鱼腥藻的早期发育产生有害影响，