We have studied the effects of hydrogen peroxide (H₂O₂) on the differentiation and maintenance of C2C12 myoblasts. The effects of H₂O₂ were evaluated by cell viability, total protein concentration, the relative amount of muscle-related proteins, sarcomere structure, and active tension generation. Oxidative stress is one of the major causes of myopathy after exercise and thus establishing the method to evaluate the effects on muscle function is essential. The primary function of striated muscle is to generate force, thus, the measurement of active tension is important in assessing the effect of chemicals on muscle. Among the indices we tested, the sarcomere structure was the most sensitive to the H₂O₂ exposure while the cell viability was less sensitive. The effects of H₂O₂ on active tension correlated with a decrease in the amount of muscle proteins. In this study, our results showed that the effect of chemicals on muscle should be measured in multiple ways, including active tension generation, for a better understanding of its physiological impact.

我们研究了过氧化氢（H ₂ O ₂）对C2C12成肌细胞分化和维持的影响。通过细胞活力，总蛋白浓度，肌肉相关蛋白的相对量，肌节结构和主动张力产生来评估H ₂ O ₂的作用。氧化应激是运动后肌病的主要原因之一，因此建立评估肌肉功能影响的方法至关重要。横纹肌的主要功能是产生力量，因此，主动张力的测量对于评估化学物质对肌肉的作用很重要。在我们测试的指标中，肌小节结构对H ₂ O最敏感_2次暴露，而细胞活力则较不敏感。H ₂ O ₂对主动张力的影响与肌肉蛋白质量的减少有关。在这项研究中，我们的结果表明，应通过多种方式来测量化学物质对肌肉的作用，包括主动产生张力，以更好地了解其生理影响。