Cyclocarya paliurus (Batal.) Iljinsk is an important medicinal plant for treating chronic diseases, but it is difficult to obtain high yields when growing on low-fertility soil. Inoculation with soil beneficial microorganism has suggested an effective means of stimulating plant growth and secondary metabolite production, but effect on plant performance when competing degraded field condition remains unclear. We combined controlled laboratory experiments with field trials to investigate the effects of co-inoculation with phyto-stimulatory strains (Azospirillum brasilense and Pseudomonas fluorescens) and nutrient-enhancing strains (Bacillus megaterium and Azotobacter chroococcum). Bacteria were applied with organic fertilizer at different fertilizer levels, and we tracked effects on soil nutrient availability as well as C. paliurus morphological traits, photosynthesis, growth and bioactive compounds during cultivation on barren land. Amendment of beneficial microbes with organic fertilizer enhanced the soil nutrient availability with high fertilizer showing greatest stimulation under controlled conditions, with the medium fertilizer giving best results in improving plant performance in the field. All fertilization regimes expanded the 3D root architecture, and bacterial additions increased the proportion of lateral roots compared to a single organic fertilizer treatment, which led to higher nutrient uptake. Inoculations at medium fertilizing level modified the root system and increased the photosynthesis rate, nutrient acquisition and plant growth. The co-inoculation with B. megaterium and P. fluorescens at medium fertilizer level stimulated the accumulation of flavonoids and polysaccharides, while co-inoculation with A. chroococcum and A. brasilense at low fertilizing level facilitated the production of flavonoids and triterpenoids. The biosynthesis of secondary metabolites exhibited strong correlations with leaf C/N and C/P ratios. Thus, manipulation of bioactive compounds in C. paliurus leaves can be affected by internal nutrient balance, which is associated with reformed root system morphology that modulated by bacterial inoculation.

Cyclocarya paliurus（Batal。）Iljinsk是治疗慢性病的重要药用植物，但在低肥力的土壤上生长时很难获得高产。土壤有益微生物的接种已表明是刺激植物生长和次生代谢产物的有效手段，但是当竞争性的退化田间条件竞争时，对植物性能的影响尚不清楚。我们将受控实验室实验与现场试验相结合，以研究共同接种植物刺激性菌株（巴西固氮螺菌和荧光假单胞菌）以及营养增强型菌株（巨大芽孢杆菌和嗜绿固氮菌）的共同作用）。将细菌与不同肥料水平的有机肥料一起施用，我们追踪了对土壤养分利用率以及C. paliurus的影响贫瘠土地上耕作过程中的形态特征，光合作用，生长和生物活性化合物。有机肥料对有益微生物的改良提高了土壤养分的利用率，在受控条件下，高肥料表现出最大的刺激作用，而中肥料在改善田间植物生长性能方面取得了最佳效果。与单一有机肥处理相比，所有施肥方案都扩大了3D根的结构，细菌的添加增加了侧根的比例，从而导致更高的养分吸收。中度施肥水平可以改变根系，提高光合作用，养分吸收和植物生长。与巨大芽孢杆菌和荧光假单胞菌的共同接种在中等肥料水平下，可促进类黄酮和多糖的积累，而在低施肥水平下与绿球藻和巴西柔毛菌一起接种可促进类黄酮和三萜的产生。次生代谢产物的生物合成表现出与叶片C / N和C / P比的强烈相关性。因此，操纵杆假单胞菌叶片中的生物活性化合物可能受到内部营养平衡的影响，内部营养平衡与通过细菌接种调节的改良根系形态有关。