A novel smartphone-based colorimetric biosensor based on enzyme-inorganic hybrid nanoflowers was applied for ultrasensitive determination of of hydrogen peroxide (H₂O₂). Horseradish peroxidase-inorganic hybrid nanoflowers (HRP-Cu₃(PO₄)₂⋅3H₂O HNFs) were facilely synthesized by a one-pot incubation using HRP and Cu₃(PO₄)₂⋅3H₂O as organic and inorganic components, respectively. Characterization results revealed HRP molecules were well immobilized in HRP-Cu₃(PO₄)₂⋅3H₂O HNFs via self-assembly. The results of smartphone-based colorimetric assay successfully demonstrated the good sensing performance of HRP-Cu₃(PO₄)₂⋅3H₂O HNFs. The linear detection range of H₂O₂ was from 5 to 500 μM, and the detection limit was 0.5 μM. Further, the proposed assay showed good anti-interference ability and high sensitivity for H₂O₂ detection owing to the high specificity of HRP and excellent accuracy of color parameters obtained by Color Detector software, respectively. In addition, HRP-Cu₃(PO₄)₂⋅3H₂O HNFs had desirable storage stability and reusability, and this colorimetric system can realize the H₂O₂ detection in tap water, which indicated the good applicability of HRP-Cu₃(PO₄)₂⋅3H₂O HNFs in real sample.

基于酶-无机杂化纳米花的新型基于智能手机的比色生物传感器被用于过氧化氢（H ₂ O ₂）的超灵敏测定。辣根过氧化物酶-无机杂化纳米花（HRP-Cu系₃（PO ₄）₂ ⋅3H ₂ öHNFs）的轻便通过一釜温育使用HRP和Cu合成₃（PO ₄）₂ ⋅3H ₂ O作为有机和无机组分， 分别。表征结果显示HRP分子以及固定在HRP-Cu系₃（PO ₄）₂ ⋅3H ₂O HNF通过自组装。基于智能手机的比色测定的结果成功地证明HRP-Cu的良好检测性能₃（PO ₄）₂ ⋅3H ₂ öHNFs。H ₂ O ₂的线性检测范围为5至500μM，检测极限为0.5μM。此外，由于HRP的高特异性和通过Color Detector软件获得的出色的颜色参数准确性，所提出的测定方法对H ₂ O _2的检测显示出良好的抗干扰能力和高灵敏度。此外，HRP-Cu系₃（PO ₄）₂ ⋅3H ₂øHNFs有希望的储存稳定性和可重用性，并且这比色系统可实现为H ₂ Ó ₂检测在自来水中，这表明HRP-Cu的良好的适用性₃（PO ₄）₂ ⋅3H ₂ öHNFs真实样本。