This study was conducted to characterize the morphology and morphometry of follicles containing multiple oocytes (MOFs) and determine the association with the FecG^E mutation in Santa Inês ewes. Based on the genotypes, 65 ewes were characterized as being homozygous wild-type (n = 25; FecG^+/+), heterozygous mutant (n = 27, FecG^+/E), and homozygous mutant (n = 13, FecG^E/E). The variables evaluated were follicle developmental stage, number of oocytes per follicle, morphology, and morphometry of MOFs. The FecG^E mutation did not affect the frequency of MOFs (P > 0.05) (3.0 % in FecG^+/+; 3.3 % in FecG^+/E; and 3.5 % in FecG^E/E). The greater viability (P < 0.05) of MOFs was identified in transitory stage of the FecG^E/E (95.0 %) and FecG^+/E (90.9 %) when compared to the FecG^+/+ genotype (73.3 %). Furthermore, the morphology of transitory follicles with two oocytes was the variable and when evaluated was the most reliable determinant for predicting which ewes had an FecG^E mutation. In conclusion, the FecG^E mutation did not affect the frequency of MOFs. The ewes with FecG^E mutation had a greater frequency of morphologically normal MOFs in the transitory stage. Furthermore, the ewes with the FecG^E mutation had a greater likelihood of having MOFs containing two morphologically normal oocytes.

本研究旨在表征含有多个卵母细胞 (MOF) 的卵泡的形态学和形态计量学，并确定与圣伊内斯母羊的FecG ^E突变的关联。基于基因型，65只母羊表征为纯合的野生型（Ñ = 25;的fECG ^{+ / +}），杂合子突变体（Ñ = 27，的fECG ^{+ / E}），以及纯合子突变体（Ñ = 13，的fECG ^{È /乙})。评估的变量是卵泡发育阶段、每个卵泡的卵母细胞数量、形态和 MOF 的形态测量。在胎儿心电^Ë突变不影响 MOF 的频率（P > 0.05）（FecG ^{+/+ 中为}3.0% ；FecG ^{+/ E 中为}3.3% ；FecG ^{E/E 中为}3.5% ）。与FecG ^+/+基因型 (73.3 %)相比，在FecG ^{E / E} (95.0 %) 和FecG ^+/E (90.9 %) 的过渡阶段确定了 MOF的更大活力 ( P < 0.05 )。此外，具有两个卵母细胞的瞬时卵泡的形态是可变的，在评估时是预测哪些母羊具有FecG ^E的最可靠的决定因素突变。总之，FecG ^E突变不影响 MOF 的频率。具有FecG ^E突变的母羊在过渡阶段具有更高频率的形态正常 MOF。此外，具有FecG ^E突变的母羊更有可能拥有包含两个形态正常的卵母细胞的 MOF。