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Occurrence, morphology, and morphometry of follicles containing multiple oocytes in FecGE mutant Santa Inês ewes
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2021-01-07 , DOI: 10.1016/j.anireprosci.2021.106690
Maiana Silva Chaves 1 , Hymerson Costa Azevedo 2 , Valesca Barreto Luz 3 , José Carlos Ferreira-Silva 1 , Inácio Barros 4 , Samuel Rezende Paiva 5 , Eduardo de Olivera Melo 6 , Deborah de Melo Magalhães Padilha 7 , Vicente José de Figueirêdo Freitas 8 , Cláudio Coutinho Bartolomeu 1 , Marcos Antônio Lemos Oliveira 1
Affiliation  

This study was conducted to characterize the morphology and morphometry of follicles containing multiple oocytes (MOFs) and determine the association with the FecGE mutation in Santa Inês ewes. Based on the genotypes, 65 ewes were characterized as being homozygous wild-type (n = 25; FecG+/+), heterozygous mutant (n = 27, FecG+/E), and homozygous mutant (n = 13, FecGE/E). The variables evaluated were follicle developmental stage, number of oocytes per follicle, morphology, and morphometry of MOFs. The FecGE mutation did not affect the frequency of MOFs (P > 0.05) (3.0 % in FecG+/+; 3.3 % in FecG+/E; and 3.5 % in FecGE/E). The greater viability (P < 0.05) of MOFs was identified in transitory stage of the FecGE/E (95.0 %) and FecG+/E (90.9 %) when compared to the FecG+/+ genotype (73.3 %). Furthermore, the morphology of transitory follicles with two oocytes was the variable and when evaluated was the most reliable determinant for predicting which ewes had an FecGE mutation. In conclusion, the FecGE mutation did not affect the frequency of MOFs. The ewes with FecGE mutation had a greater frequency of morphologically normal MOFs in the transitory stage. Furthermore, the ewes with the FecGE mutation had a greater likelihood of having MOFs containing two morphologically normal oocytes.



中文翻译:

FecGE 突变体圣伊内斯母羊中含有多个卵母细胞的卵泡的发生、形态和形态测量

本研究旨在表征含有多个卵母细胞 (MOF) 的卵泡的形态学和形态计量学,并确定与圣伊内斯母羊的FecG E突变的关联。基于基因型,65只母羊表征为纯合的野生型(Ñ = 25;的fECG + / +),杂合子突变体(Ñ = 27,的fECG + / E),以及纯合子突变体(Ñ = 13,的fECG È /)。评估的变量是卵泡发育阶段、每个卵泡的卵母细胞数量、形态和 MOF 的形态测量。在胎儿心电Ë突变不影响 MOF 的频率(P > 0.05)(FecG +/+ 中为3.0% ;FecG +/ E 中为3.3% ;FecG E/E 中为3.5% )。与FecG +/+基因型 (73.3 %)相比,在FecG E / E (95.0 %) 和FecG +/E (90.9 %) 的过渡阶段确定了 MOF的更大活力 ( P < 0.05 )。此外,具有两个卵母细胞的瞬时卵泡的形态是可变的,在评估时是预测哪些母羊具有FecG E的最可靠的决定因素突变。总之,FecG E突变不影响 MOF 的频率。具有FecG E突变的母羊在过渡阶段具有更高频率的形态正常 MOF。此外,具有FecG E突变的母羊更有可能拥有包含两个形态正常的卵母细胞的 MOF。

更新日期:2021-02-07
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