This study was conducted to determine the occurrence of E. multilocularis in foxes and environmental fecal contamination by E. multilocularis in Erzurum, the most highly endemic region for AE in Turkey. The study materials consisted of 50 red fox carcasses collected from 20 counties of Erzurum, Turkey, between October 2015 and February 2016. After the application of the sedimentation and counting technique (SCT), E. multilocularis was identified through the identification of typical morphological structures. Fox fecal samples (n = 600) were also collected from these counties for the isolation of taeniid eggs using the sequential sieving and flotation method (SSFM). Then, the collected adult worms and taeniid eggs were subjected to molecular and sequence analyses. Mature E. multilocularis parasites were found in 42% (21/50) of the fox intestines, with a mean number of 7,806 (150–31,644). The severity of infection was higher in carcasses obtained from the central district (48.6%, 17/35) than in those obtained from the peripheral district (26.7%, 4/15). The prevalence of environmental fecal contamination with E. multilocularis was 10.5% (63/600) in fecal samples collected from all counties of Erzurum. This infection rate was higher in the central district (32.1%, 36/112) than in the peripheral district (5.5%, 27/488; P < 0.0001). In conclusion, contrary to expectation, the prevalence of E. multilocularis positivity was high in urban areas. This could have been due to alterations in the dietary habitats of definitive and intermediate hosts. Therefore, new control strategies are essential to eliminate human AE cases in the future as urbanization advances.

这项研究的目的是确定在土耳其AE的最高流行地区Erzurum中，狐狸中多眼大肠杆菌的发生和环境粪便对多眼大肠杆菌的污染。研究材料由2015年10月至2016年2月之间从土耳其埃尔祖鲁姆20个县收集的50只赤狐car体组成。应用沉降计数技术（SCT）后，多叶大肠杆菌通过鉴定典型的形态结构来鉴定。还从这些县收集了福克斯粪便样本（n = 600），使用顺序筛分和浮选法（SSFM）分离了eni虫卵。然后，对收集的成虫和虫卵进行分子和序列分析。在狐狸肠中有42％（21/50）发现了成熟的多眼大肠杆菌寄生虫，平均数量为7,806（150-31,644）。从中心区获得的尸体的感染严重程度更高（48.6％，17/35），而从周围地区获得的尸体（26.7％，4/15）更高。多粪肠球菌对环境粪便污染的流行从埃尔祖鲁姆所有县收集的粪便样品中的粪便中的总胆固醇含量为10.5％（63/600）。该感染率在中部地区（32.1％，36/112）高于外围地区（5.5％，27/488; P <0.0001）。总之，与预期相反，城市地区的多眼肠埃希菌阳性率很高。这可能是由于最终宿主和中间宿主的饮食生境发生了变化。因此，随着城市化进程的发展，新的控制策略对于将来消除人类AE病例至关重要。